OBJECTIVE To study the clinical outcomes of single frozen-thawed blastocyst transfer cycles according to the hatching status of frozen-thawed blastocysts. METHODS Frozen-thawed blastocysts were divided into three groups according to their hatching status as follows: less-than-expanded blastocyst (≤EdB), hatching blastocyst (HgB), and hatched blastocyst (HdB). The female age and infertility fa...

BACKGROUND As embryo selection is not allowed by law in Switzerland, we need a single early scoring system to identify zygotes with high implantation potential and to select zygotes for fresh transfer or cryopreservation. The underlying aim is to maximize the cumulated pregnancy rate while limiting the number of multiple pregnancies. METHODS In all, 613 fresh and 617 frozen-thawed zygotes wer...

Objectives: To compare the clinical outcomes of single fresh and frozen-thawed superior blastocyst transfer in patients for the first transfer. Methods: This study included 770 single fresh blastocyst and 639 single frozenthawed blastocyst transfers from January 2013 to January 2016. All the fresh and frozen-thawed embryo transfer was first transfer for the patients. The patients were divided i...

BACKGROUND Artificial insemination (AI) using frozen-thawed semen is well established and routinely used for breeding in various mammalian species. However, there is no report of the birth of elephant calves following AI with frozen-thawed semen. The objective of the present study was to investigate the fertilizing ability of chilled and frozen-thawed semen in the Asian elephant following artif...

Fresh and post-thaw parameters (motility, morphology and viability) of stallion epididymal spermatozoa that have been and have not been exposed to seminal plasma were evaluated, and directly compared to fresh and post-thaw parameters of ejaculated spermatozoa. Six sperm categories of each stallion (n=4) were evaluated for motility, morphology and viability. These categories were fresh ejaculate...

Semen from six adult male Black Bengal goats (Capra hircus) was collected to compare the fresh and frozen-thawed semen quality and investigate its relationship with the fertility. Each collected samples was divided into two parts: One part of semen was used as fresh and another part was filled into 0.50 ml straws, sealed, cooled (5 ˚C) and equilibrated for freezing.In both cases, semen was dilu...

The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...

background: frozen- thawed embryo transfer is an essential part of art treatment and outcomes of this procedure are associated with several clinical factors. several studies have showed an increase level of ivf outcomes in women with sufficient vitamin d. objective: whether treatment of vitamin d insufficiency can improve pregnancy rates in frozen-thawed embryo transfer cycles. materials and me...

OBJECTIVE To evaluate if the outcomes of IVF/ICSI in frozen-thawed embryo transfer and fresh embryo transfer cycles differ in relation to cleavage and blastocyst stages. METHODS Retrospective cohort study to compare IVF/ICSI outcomes between fresh embryo transfer and frozen-thawed embryo transfer cycles, according to the stage of embryo development. Analysis was carried out on 443 consecutive...

This retrospective consecutive case series aimed at comparing the results of intracytoplasmic sperm injection (ICSI) with fresh and with frozen-thawed epididymal spermatozoa obtained after microsurgical epididymal sperm aspiration (MESA) in 162 couples. These couples were suffering from infertility because of congenital bilateral absence of the vas deferens (n = 109), failed microsurgical rever...