BACKGROUND Although mouse spermatozoa can be freeze-dried without losing their reproductive capacity, the technique needs further improvements to reduce the incidence of chromosomal damage to spermatozoa. Effects of freeze-drying on human spermatozoa are unknown. METHODS Mouse spermatozoa were suspended in a Tris-buffered EGTA solution briefly (10 min at 37 degrees C) or for 1-7 days at 4 deg...

We compared hepatitis B virus (HBV) surface antigen, anti-hepatitis C virus (HCV) antibody, and HCV RNA quantification in frozen and freeze-dried serum samples to assess the usefulness of freeze-dried sera for detection of HBV and HCV. The results indicated that freeze-dried sera as well as frozen sera can be useful for serological and molecular biological analyses of HBV and HCV.

Freeze-dried bone allograft is an interesting treatment alternative to autogenous bone grafts. This clinical report presents a 3-year follow-up of an atrophic maxilla treated with freeze-dried bone allograft. Ridge augmentation was conducted with freeze-dried tibial allografts. Eight implants were used to support a full-arch prosthesis. Three years later, clinical and radiographic follow-up sho...

Groups of 30 rats each were fed 1 of 4 diets to determine feeding preference for freeze-dried meal compared to dry meal, and to determine if 3% agar is a desirable component of freeze-dried meal. The diets were: dry meal, meal + distilled water(freeze-dried), meal + 3% agar in distilled water(freeze-dried), and meal + 3% agar in distilled water(gel). Diet preparation, and the method for introdu...

Autogenous bone graft is gold standard in treating bone defects, but it might have difficulty in corporation and rejection reaction. This study is to compare the effectiveness among freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, and demineralized bone matrix xenograft as bone graft to fill bone defect in femoral diaphysis of white rabbit. Thirty male New Zealand white...

Freeze-dried mouse spermatozoa can be used for normal embryonic development after injection into oocytes, thus indicating that freeze-drying is a useful method for the storage and transportation of genetic materials from animals. We recently reported that storage of freeze-dried mouse spermatozoa requires maintenance at temperatures lower than -80 C for long-term preservation and a pressure of ...

Cold pressed peel oil was added to high quality 50°Brix commercial orange concentrate and foam-mat dried, or diluted to single strength juice and freeze-dried using different platen temperatures. Amounts of peel oil ranged from 0.026% to 0.106% for samples to be foam-mat dried, and from 0.011% to 0.109% for freeze drying. For foam-mat drying, previously de veloped standard commercial conditions...

Bovine viral diarrhoea virus 1 (BVDV-1) is arguably the most important viral disease of cattle. It is associated with reproductive, respiratory and chronic diseases in cattle across the world. In this study we have investigated the capacity of the major immunological determinant of BVDV-1, the E2 protein combined with hollow type mesoporous silica nanoparticles with surface amino functionalisat...

This study evaluates the nutritional value of several species of freeze-dried microalgae used to feed the seed of Ruditapes decussatus. These microalgae were: Isochrysis galbana, clone T-ISO; Tetraselmis suecica; and Phaeodactylum tricornutum. The addition of antibiotics, kaolin and fresh microalgae to the freeze-dried diet was also evaluated, and a study was made of the nutritional value of on...