Researchers add serum to a classical medium at concentrations of 5 to 10% (v/v) to grow cellsin-vitro culture media. Unfortunately, serum is a poorly defined culture medium componentas its composition can vary considerably while serum-free cell culture media are an excellentalternative to standard serum-containing media and offer several major advantages. Advantagesof us...

An increase in the flow velocity in flowthrough large porous media and deflection of flow regime from Darcy law causes a nonlinear relationship between hydraulic gradient and pore velocity of media. So many investigations have done previously in such Medias. One of the most important subjects in flowthrough large porous media is relationship between friction coefficient and flow characteristics...

BACKGROUND Mesenchymal stem cells are capable of self-renewal and multi-lineage differentiation. They are used extensively to treat several diseases. Traditionally, mesenchymal stem cells are cultured in serum-containing media, typically supplemented with fetal bovine serum (FBS). However, the variability of FBS is likely to skew experimental results. Although serum-free media used to expand me...

in recent years, many researchers have been done in the area of wireless sensornetworks that has led to the variety of algorithms and methods for the collection andtransmission of environment information; but still many challenges faced bywireless sensor networks using methodologies are applied in various fields. such asmedia access control that due to the media being shared on these networks, ...

human peripheral blood monocytes (hpbm) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. after a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. paralleli...

Use of serum-free media and the related genera of animal-free and protein-free media has increased significantly over the past 15 years. This is particularly true in industrial applications, in which the use of serum presents a safety hazard and a source of unwanted contamination in the production of biopharmaceuticals (1). As the name suggests, serum-free media are prepared without animal seru...

Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. However, there are serious scientific and ethical concerns about the use of FBS regarding its harvest and production. During the last three decades, FBS could be substituted by other supplements or by the use of defined chemical components in serum-free cell culture. A number of serum-free medium formula...