The aim of this study was to investigate Fragile Histidine Triad Gene (FHIT) expression in laryngeal squamous cell carcinoma. The paraffin embedded tissue blocks of 64 laryngeal squamous cell carcinoma specimens were enrolled in the study. FHIT expression was detected by an immunohistochemical method. Rabbit polyclonal antibody was used for immunohistochemical study. FHIT expression was low in ...

The histidine triad proteins (HITs) constitute a large and ubiquitous superfamily of nucleotide hydrolases. The human histidine triad nucleotide-binding proteins (hHints) are a distinct class of HITs noted for their acyl-AMP hydrolase and phosphoramidase activity. The first high-resolution crystal structures of hHint2 with and without bound AMP are described. The differences between hHint2 and ...

Fragile histidine triad (HIT) proteins (Fhits) occur in all eukaryotes but their function is largely unknown. Human Fhit is presumed to function as a tumour suppressor. Previously, we demonstrated that Fhits catalyse hydrolysis of not only dinucleoside triphosphates but also natural adenosine 5'-phosphoramidate (NH2-pA) and adenosine 5'-phosphosulfate (SO4-pA) as well as synthetic adenosine 5'-...

Abbreviations: GBC: Gallbladder Cancer; PCR: Polymerase Chain Reaction; SSCP: Single Strand Conformation Polymorphism; MSI: Microsatellite Instability; CIS: Carcinoma In Situ; AJPBD: Anomalous Junction of Pancreatico Biliary Duct; FHIT: Fragile Histidine Triad; CC: Chronic Cholecystitis; XGC: Xanthogranulomatous Cholecystitis; SAGE: Serial Analysis of Gene Expression; CTGF: Connective Tissue Gr...

Human platelets diadenosine triphosphatase was characterised and compared with the Fhit protein, a human tumour suppressor with diadenosine triphosphatase activity. Both enzymes exhibit similar Km, are similarly activated by Mg2+, Ca2+ and Mn2+, and inhibited by Zn2+ and suramin. However, they are differentially inhibited by Fhit antibodies and exhibit differences in gel-filtration behaviour.

The aliphatic amidase from Pseudomonas aeruginosa belongs to the nitrilase superfamily, and Cys(166) is the nucleophile of the catalytic mechanism. A model of amidase was built by comparative modelling using the crystal structure of the worm nitrilase-fragile histidine triad fusion protein (NitFhit; Protein Data Bank accession number 1EMS) as a template. The amidase model predicted a catalytic ...