Obesity and obesity co-morbidities are associated with a low grade inflammation and elevated serum levels of acute phase proteins, including serum amyloid A (SAA). In the non-acute phase in humans, adipocytes are major producers of SAA but the function of adipocyte-derived SAA is unknown. To clarify the role of adipocyte-derived SAA, a transgenic mouse model expressing human SAA1 (hSAA) in adip...

bacteriorhodopsin operates as a light/proton transfer pump which converts the light energy into a proton gradient. energy stored in the proton gradient can be used in a variety of ways. bacteriorhodopsin producing sources are some halobacterium species such as halobacterium sodomense and halobacterium salinarum which continue to grow in harsh and salt-saturated conditions. two types of red pigm...

Impaired secretion of lithostathine, a pancreatic glycoprotein capable of inhibiting the growth of CaCO3 crystals, has been reported in chronic calcifying pancreatitis. Controversial results were obtained, however, using immunoassays with different antibodies. The aim of this study was to purify and to measure juice lithostathine by a non-immunological method. Fast protein liquid chromatography...

Brugia malayi microfilarial excretory-secretory (mf ES) and phosphate buffer saline soluble (mf S) antigens were fractionated by fast protein liquid chromatography (FPLC) on superdex 200 HR 10/30 gel filtration column. The active antigen fractions were identified and explored in comparison with whole mf ES and mf S antigens to detect filarial IgG antibodies in different groups viz microfilaraem...

Abstract Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols been reported the isolation of EVs with preserved biological function. Most EV purification methods include a precipitation step that results in aggregation most available techniques do not efficiently separate various types such as exosomes ect...

We present here an improved RNA purification method using fast performance liquid chromatography (FPLC) size-exclusion chromatography in place of denaturing polyacrylamide gel electrophoresis (PAGE). The method allows preparation of milligram quantities of pure RNA in a single day. As RNA oligonucleotides behave differently from globular proteins in the size-exclusion column, we present standar...

A new toxic protease was isolated from Aeromonas salrnonicida subspecies achromogenes extracellular products (ECP). It was detected in 5 of 9 strains of the organism tested. The protease was purified by anion-exchange fast protein liquid chromatography (FPLC) on Mono Q HR 5/5, and FPLC gel filtration on Superose 12 HR 10/30. The LDso of the purified protease for juvenile Atlantic salmon Salrno ...

Antibadi moleküllerinin proteolitik enzimlerle alt birimlerine parçalanabildiği bilinmektedir. Bu alt birimler, antibadilerin yapısı ve işlevlerinin belirlenmesi çalışmalarında önemli veriler sunmaktadır. Deneysel uygulamalarda ve immünokimyasal teknikler için antibadi alt birimleri, parçalanmamış bütün antibadi moleküllerine göre önemli avantajlar sunmaktadır. Alt birimlerin boyutları daha küç...