Epiberberine (EPI) is a novel and potentially effective therapeutic and preventive agent for diabetes and cardiovascular disease. To evaluate its potential value for drug development, a specific, sensitive and robust high-performance liquid chromatography-tandem mass spectrometry assay for the determination of EPI in rat biological samples was established. This assay was used to study the pharm...

recently, incremental sheet metal forming (ismf) process as one of the new methods in rapid prototyping collection has been considered by researchers. this process is based on a defined path in cnc controller and applies a spherical-head tool that supplies the required pressure for sheet metal forming. despite affirmation of process ability in forming symmetric geometries, some of its aspects s...

Background: Inhibition of the androgen receptor (AR) is mainstay treatment for advanced prostate cancer. While initially effective, disease ultimately progresses to metastatic castration-resistant cancer (mCRPC), which lethal. All current AR-targeted therapies directly or indirectly target ligand binding domain (LBD). We have developed EPI and sintokamide (SINT) compounds AR N-terminal (NTD), a...

Okadaic acid (OKA) and analogues are frequent contaminants of coastal waters and seafood. Structure analysis of the isolated OKA analogue 19-epi-OKA showed important conformation differences expected to result in lower protein phosphatase (PP) inhibitory potencies than OKA. However, 19-epi-OKA and OKA inhibitory activities versus PP2A were unexpectedly found to be virtually equipotent. To inves...

To determine the effects of an increase in lipolysis on the glycogenolytic effect of epinephrine (EPI), the catecholamine was infused portally into 18-h-fasted conscious dogs maintained on a pancreatic clamp in the presence [portal (Po)-EPI+FFA, n = 6] and absence (Po-EPI+SAL, n = 6) of peripheral Intralipid infusion. Control groups with high glucose (70% increase) and free fatty acid (FFA; 200...

Anthracyclines are effective genotoxic anticancer drugs for treating human malignancies; however, their clinical use is limited by tumor resistance and severe cardiotoxicity (e.g., congestive heart failure). Epirubicin (EPI) is less cardiotoxic compared with other canonical anthracyclines (e.g., doxorubicin). This has been attributed to its unique glucuronidation detoxification pathway. EPI is ...

Introduction: Low frequency fluctuations (LFFs) in T2*-weighted MR images have been used to map functional connectivity in both humans and rats [1, 2, 3]. Most FC studies utilize gradient echo EPI (GE-EPI) because of its high sensitivity to BOLD signal fluctuations. However, fMRI studies have shown that the BOLD signal in GE-EPI images contains a strong contribution from large veins. At high fi...

The rate of hepatic glucose production (R(a) glucose) of rainbow trout (Oncorhynchus mykiss) was measured in vivo by continuous infusion of [6-(3)H]glucose and in vitro on isolated hepatocytes to examine the role of epinephrine (Epi) in its regulation. By elevating Epi concentration and/or blocking beta-adrenoreceptors with propranolol (Prop), our goals were to investigate the mechanism for Epi...

Epirubicin (EPI), an anthracycline antitumour antibiotic, is a known intercalating and DNA damaging agent. Here, we study the molecular interaction of EPI with histones and other cellular targets. EPI binding with histone core protein was predicted with spectroscopic and computational techniques. The molecular distance r, between donor (histone H3) and acceptor (EPI) was estimated using Förster...

The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications (e.g., brightfield, phase-contrast, differential interference contrast, and fluorescence epi-illumination), and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The principles of...