نتایج جستجو برای: followed by centrifugation

تعداد نتایج: 7082409  

Journal: :iranian biomedical journal 0
سعید بوذری saeid bouzari veladimir o. rechinsky

dna polymerase gene from thermus aquaticus strain yt1 was amplified using venttm dna po-lymerase and cloned under the control of x.pr promoter and expression was induced by a shift in tern perature. the culture was then sonicated, and after centrifugation the lysate was treated with poly ethyleneimine followed by a salting-out step. finally the protein was precipitated with ammonium sulfate and...

پایان نامه :وزارت علوم، تحقیقات و فناوری - پژوهشگاه پلیمر و پتروشیمی ایران - پژوهشکده پتروشیمی 1389

this is an original study on the effect of a polyisobutylene – polydimethylsiloxane (pibpdms) block copolymer modifier used as an interfacial active agent on the dynamics behaviour of single newtonian drops suspended in a polyisobutylene (pib) newtonian matrix. the results were divided in two sections. the first part included the experiments carried out on the non-modified and 2% block cop...

Journal: :Journal of nematology 1990
N Greco T D'Addabbo

Investigations were undertaken to determine the suitability of sucrose and magnesium sulphate solutions and a silica colloidal suspension with centrifugation for extracting Tylenchulus semipenetrans from citrus roots. The efficiency of incubation, sodium hypochlorite, centrifugation, and maceration methods was also compared. Numbers of females recovered by centrifugation with colloidal silica w...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد یزد - دانشکده شیمی 1392

in this study, a simple, rapid and selective method was developed for the determination of dexamethasone. the proposed method is based on inhibitory effect of dexamethasone on the oxidation of orange-g by bromate in sulfuric acid media. the reaction was followed spectrophotometrically at 478.5 nm (?max). under optimum experimental conditions, (72.6 ?mol l-1 of orange-g, 0.76 mol l-1 of h2so4, 0...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد گرمسار - دانشکده علوم انسانی 1390

this study was an attempt to investigate the effect of subtitling on vocabulary learning among iranian intermediate students. to find the homogeneity of the groups, tofel test was administered to student in kish mehr institute in garmsar. after analyzing the data, 60 participants (female students) who scored within the range of one standard deviation above and below the mean, were selected as h...

Journal: :School Science and Mathematics 1922

Journal: :Applied microbiology 1971
I Toplin M Mizell P Sottong J Monroe

A series of "winter" and "summer" Lucké kidney tumors of the frog (Rana pipiens) were homogenized and fractionated by differential centrifugation into nuclear, mitochondrial, and mitochondrial supernatant fractions. Winter tumors often contained high concentrations of herpesvirus, whereas no virus was observed in any of the summer tumors. The crude tumor fractions were further purified by rate-...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه اراک - دانشکده علوم انسانی 1389

abstract this study examines the relationship between reading anxiety and difficulty of texts as well as the relationship between reading anxiety and students perceived difficulty of the texts. since difficulty is a relative concept, i limited its definition by sticking to the readability formula. we also took students perceived difficulty levels into account. therefore, in the present study, ...

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