Although protein is the third most abundant solid in bile and is important in cholesterol crystal formation, methods for quantitating the concentration of total protein in bile have not been systematically evaluated. To establish a reliable protein assay for bile, we evaluated three protein assays (Lowry's method and the fluorescamine and Coomassie blue methods), and employed amino acid analysi...

penicillin acylase (ec 3.5.1.11) has been a target of study for a long time because of its pivotal role in the deacylation of the penicillin into the 6- aminopenicillanic acid (6-apa) and the side-chain organic acids. this property of penicillin acylase has been exploited commercially for large scale production of 6-apa, which is the key intermediate in the manufacture of semi-synthetic penicil...

We describe a fluorometric procedure, with use of fluorescamine, for determining plasma amino nitrogen in a 10-mul sample of deproteinized plasma. Values for apparently healthy human subjects agree well with those obtained by spectrophotometry. The simplicity and speed of the assay recommends it as a screening procedure for detecting aminoacidopathies.

In this study, the fluorogenic efficiency of fluorescamine for spectrofluorimetric analysis of primary amines was investigated. Different mixtures of dichloromethane and other organic solvents were used for evaluation of fluorescence stability and intensity of fluorescamine derivative (Pyrrolinone). Solvents such as acetonitrile (ACN), tetrahydrofuran (THF) and dioxane increase stability an...

The labeling of the protein moiety of the sarcoplasmic calcium transport ATPase by fluorescamine suppresses calcium transport, calcium dependent ATPase activity, protein phosphorylation by [gamma-32P]ATP and [32P]phosphate at different extent of amino group substitution. For the hydrolysis of para nitrophenylphosphate by the calcium transport ATPase, it is shown that the relationship between th...

in this study, the fluorogenic efficiency of fluorescamine for spectrofluorimetric analysis of primary amines was investigated. different mixtures of dichloromethane and other organic solvents were used for evaluation of fluorescence stability and intensity of fluorescamine derivative (pyrrolinone). solvents such as acetonitrile (acn), tetrahydrofuran (thf) and dioxane increase stability and in...

A method is described for determining the long-chain base content of glycosphingolipids after acid hydrolysis, using the new reagent fluorescamine. The reaction is sensitive and can be used to characterize or measure glycosphingolipids in quantities routinely separated by thin-layer chromatography.

Aims: To synthesize small molecule alkylating compounds and analyze the kinetics of the alkylation in aqueous solution. Determine molecular properties and the drug likeness of these four compounds as potential antineoplastic agents and apply statistical analysis to identify interrelationships of properties. Study Design: Four compounds were synthesized, characterized, and studied for alkylation...

An isocratic reversed-phase high-pressure liquid chromatographic method for the determination of six components of the teicoplanin complex in biological fluid was developed. By using fluorescence detection after precolumn derivatization with fluorescamine, the assay is specific and highly sensitive, with reproducibility studies yielding coefficients of variation ranging from 1.5 to 8.5% (at 5 t...