a one kb portion of the rpos gene from flexibacter chinensis was isolated by pcr, sequenced and compared to the rpos gene of a variety of other organisms. the gene was found to be 98% similar to previously sequenced genes. mutation of the rpos gene with tri-parental mating produced strain jr101 and the growth rate of the mutant was compared with that of the wild-type. the mutant grew slower, an...

We found a new inhibitor of mammalian DNA topoisomerase I, named topostin, from a bacterial culture. The bacteria was identified as Flexibacter topostinus sp. nov., B-572. Morphological and physiological characteristics, and utilization of sugars were examined. Comparison of the strain with known species of the genus Flexibacter was made and indicates that the strain is a new species of the gen...

Bacterial strains were isolated from sponge and green algae which were collected on the coast of Japan and Palau. The phylogenetic relationships of these isolates among marine species of the Cytophaga-Flavobacterium-Bacteroides complex were analysed by using their gyrB nucleotide sequences and translated peptide sequences (GyrB) in addition to 16S rDNA sequences. These isolates were closely rel...

Recently, Mouriño et al. (2008) reported the first record in Brazil of the filamentous bacteria Flexibacter maritimus (Wakabayashi et al., 1986) from the hatching of Litopenaeus vannamei post-larvae (Boone, 1931). In our view, a generalized lack of clarity about this identification has occurred and the discussion of some issues raised in the work is beyond the scope of this commentary. As Mouri...

Analysis of the 16S rRNA gene sequences of species currently assigned to the genus Flexibacter has shown extensive intrageneric phylogenetic heterogeneity. It has been shown in previous studies that the species [Flexibacter] sancti, [Flexibacter] filiformis and [Flexibacter] japonensis were most closely related to Chitinophaga pinensis. In addition, [Cytophaga] arvensicola and species of the ge...

A rapid diagnostic method for fry mortality syndrome is described. The method is based on immunofluorescence analysis of spleen imprints from diseased rainbow trout fry and utilizes rabbit antlserum prepared against Flexibacter psychrophilus. A low level of cross reactivity with Flexibacter columnans was observed but this can be avoided by absorbing the diagnostic antiserum with F columnans. Du...

Background: Coptis chinensis (C. chinensis) contains various antioxidants, including berberine, epiberberlin, ferulic acid, magnoflorine, palmatine, and worenine, which have antibacterial, anti-inflammatory, haemostatic, hypotensive, and anticancer effects. In the present study, the melanogenesis-inhibiting effects of C. chinensis were investigated and the molecular mechanisms were elucidated. ...

S5 File. Supporting Tables. Table A. The expression profiles of all expressed genes in developing fruit of A. chinensis cv. 'Hongyang'. Table B. List of novel transcripts, alternative splicing and genes extended identified through analysis of the 'Hongyang' transcriptome. Table C. Verification of RNA-seq results by qPCR in A. chinensis cv. 'Hongyang'. Table D. Differentially expressed genes bet...