A new high-sensitivity radiochromic film has been tested for its polarization properties. Gafchromic HS film has been shown to produce a relatively small (less than 3%) variation in the optical density measured at 660 nm wavelength when the light source is fully linear polarized and the film is rotated through a 360 angle. Similar variations are seen when the detector is linearly polarized. If ...

Chemiluminescent Western blotting is a popular protein detection method for studying proteins. The typical protocol for measuring chemiluminescent signal exposes the Western blot to laboratory-grade X-ray film, but digital imaging using a cooled-CCD camera is proving to be a more functional choice for researchers. The Thermo Scientific MYECL Imager offers digital imaging with a number of improv...

Regeneration of the dura mater following duraplasty using a collagen film, chitosan or combination both with gelatin, was studied in craniotomy and penetrating brain injury model rats. Collagen autofluorescence regenerated evaluated confocal microscopy excitation at λem = 488 nm 543 nm. An increase regeneration extracellular matrix connective tissue an fluorescence were detected 6 weeks after ...

PURPOSE This study evaluates the correlation between corneal densitometry and epithelial ingrowth (EI) after laser in situ keratomileusis (LASIK). METHODS Corneal densitometry of 3 patients who developed EI after LASIK was measured with the Oculus Pentacam. Corneal densitometry readings of each patient were obtained preoperatively and postoperatively after ingrowth was discovered. Densitometr...

An RT-PCR protocol for the relative quantitative measurement of TrkB transcripts using glyceraldehyde-3-phosphate-dehydrogenase (GAPD) transcripts as an internal control is described. Both TrkB and GAPD sequences were co-amplified in the exponential phase of amplification using 5'-biotinylated primers. The PCR products were subjected to PAGE, electro-transferred to nylon membrane and detected b...

Today, the economic consumption of biodegradable polymers is of capital importance in many applications. One of the most commonly used biopolymers is polylactic acid (PLA). The printability of the biodegradable film has not been fully investigated. This work tested the printability of polylactic acid (PLA) films and compared the print qualities to common packaging films like low density pol...

PURPOSE Allergic conjunctivitis (AC) has been reported to induce the instability of the tear film. The tear protein and the lipid layer play important roles in maintaining the tear film. The aim of this study was to quantify the alteration of the major tear protein components and a lipid related protein secretory type IIa phospholipase A2 (sPLA2-IIa) in tears of seasonal allergic conjunctivitis...

Spot density is commonly used as an indication of shower energy in emulsion chambers. In a system originally developed for JACEE analysis, the optical density of a spot on x-ray film is estimated from macroscopic digital images. The spot’s size is used to compensate for the lack of dynamic range obtainable with digital imaging hardware. These densities are compared to manually measured densities.