How intracellular transport controls the probability that cargos switch at intersections between filaments is not well understood. In one hypothesis some motors on the cargo attach to one filament while others attach to the intersecting filament, and the ensuing tug-of-war determines which filament is chosen. We investigate this hypothesis using 3D computer simulations, and discover that switch...

The nucleotide turnover rates of bipolar myosin thick filament along which actin filament slides were measured by the displacement of prebound fluorescent ATP analog 2'(3')-O-[N-[2-[(Cy3)]amindo]ethyl] carbamoyl]-adenosine 5' triphosphate (Cy3-EDA-ATP) upon flash photolysis of caged ATP. The fluorescence of the thick filament where actin filament slides decayed with two exponential processes. T...

We determine the inviscid dynamics of a point vortex in the vicinity of a flexible filament. For a wide range of filament bending rigidities, the filament is attracted to the point vortex, which generally moves tangentially to it. We find evidence that the point vortex collides with the filament at a critical time, with the separation distance tending to zero like a square root of temporal dist...

During cell locomotion and endocytosis, membrane-tethered WASP proteins stimulate actin filament nucleation by the Arp2/3 complex. This process generates highly branched arrays of filaments that grow toward the membrane to which they are tethered, a conflict that seemingly would restrict filament growth. Using three-color single-molecule imaging in vitro we revealed how the dynamic associations...

The disturbances of a quiescent filament and associated evolution of photospheric magnetic field were examined. The whole filament was broken into three sections by two filament breaks. In different sections, three distinct disturbances took place: two major ones with similar characteristics and a transient one. The presence of filament twist and the highly sheared filament magnetic field were ...

Adhesion of motile flagella to the cell body in Trypanosoma brucei requires a filamentous cytoskeletal structure termed the flagellum attachment zone (FAZ). Despite its essentiality, the complete molecular composition of the FAZ filament and its roles in FAZ filament assembly remain poorly understood. By localization-based screening, we here identified a new FAZ protein, which we called FAZ2. K...

topical application with 100, 200 and 300 ng/ml phytohormone salicyclic acid on commercial traits was analysed in bivoltine csr2, csr4 and csr2xcsr4 crossbreed races of the silkworm, bombyx mori l. the results showed that there was significant increase in larval weight female cocoon weight, male cocoon shell weight, and hatching percentage with 100 and 200 ng/ml, silkgland weight, female cocoon...

RecA protein catalyzes DNA strand exchange, a basic step of homologous recombination. Upon binding to single-stranded DNA (ssDNA), RecA protein forms a helical nucleoprotein filament. Normally, this nucleoprotein filament binds double-stranded DNA (dsDNA) and promotes exchange of base pairs between this dsDNA and the homologous ssDNA that is contained within this filament. Here, we demonstrate ...

Remodeling of actin filament arrays in response to biotic and abiotic stimuli is thought to require precise control over the generation and availability of filament ends. Heterodimeric capping protein (CP) is an abundant filament capper, and its activity is inhibited by membrane signaling phospholipids in vitro. How exactly CP modulates the properties of filament ends in cells and whether its a...