Although there is evidence that the toxic effects of cis-diamminedichloroplatinum(II) (cisplatin) include morphologically abnormal mitochondria, direct demonstrations of mitochondrial DNA damage by this chemotherapeutic agent have rarely been reported. Here we show that, in rats exposed to a single dose of cisplatin during gestation, cisplatin-DNA binding levels in both maternal and fetal liver...

In the present study, we examined the direct cytotoxic effects of cocaine on fetal cardiac myocytes. Cocaine treatment of cultured fetal rat (21 days) myocardial cells (FRMCs) induced a time- and concentration-dependent increase in apoptotic cells in FRMCs. Cocaine induced surface exposure of phosphatidylserine in FRMCs at 12-h treatment and increased apoptotic cells up to 96 h. Corresponding D...

To date, studies on the effects of prenatal exposure to diphenylamine developing fetuses are sparse. Therefore, further investigation is required determine potential hazard this compound and introduce possible treatment for these hazards. This study aimed assess biochemical, histopathological, ultrastructural changes induced by in liver kidney rat role vitamin E alleviating changes. Fifty pregn...

Mitochondria from the 7777 hepatoma incorporate substantial amounts of l-[U-(14)C]serine into phospholipid by a Ca(2+)-dependent base-exchange reaction. This reaction is virtually absent in normal liver mitochondria. The finding cannot be attributed to microsomal contamination of the sucrose gradient-purified 7777 hepatoma mitochondria. The reaction is also absent in the rapid-growth controls, ...

Mitochondria and microsomal membranes were prepared from normal, fetal, and regenerating rat liver and from seven Morris hepatomas of varying growth rates and analyzed for percentage of phospholipid composition and phospholipid con tent per mg protein. The Morris hepatomas studied included the7777,5132tc,7800,7794A,7787,9633,and 96i8A. Cardiolipin is localized in mitochondria in all tissues stu...

In order to study the aging changes of intramitochondrial DNA synthesis of mouse adrenocortical cells, 5 groups of developing mice, each consisting of 3 individuals, total 15, from fetal day 19 to postnatal day 1, 3, 9 and 14 were injected with 3H-thymidine, sacrificed 1 hr later and the adrenal tissues were processed for electron microscopic radioautography. On EM radioautograms obtained from ...

Objective. To determine the correlation between maternal bile acid (BA) level and fetal pulmonary surfactant in rats and study the effects of BA on fetal lung in rat model of intrahepatic cholestasis of pregnancy. Methods. Forty pregnant rats were treated with (A) 5.5 mg/kg BA, (B) 1.4 mg/kg BA, and (C) 1 ml physiological saline. Levels of total bile acid (TBA), ALT, AST, TBIL, DBIL, and SP-A w...

BACKGROUND Endothelial cell damage is an important pathophysiological step of restenosis after angioplasty and stenting. Cell transplantation has great therapeutic potential for endothelial recovery. We investigated the effect of transplanting endothelial progenitor cells (EPCs) derived from human early fetal aortas in rat injured arteries. METHODS The carotid arterial endothelium of Sprague-...

As development proceeds from the embryonic to fetal stages, cardiac energy demands increase substantially, and oxidative phosphorylation of ADP to ATP in mitochondria becomes vital. Relatively little, however, is known about the signaling mechanisms regulating the transition from anaerobic to aerobic metabolism that occurs during the embryonic period. The main objective of this study was to tes...