Different control strategies of bed temperature and moisture were investigated using various inlet air temperatures and air fluxes in both the ordinary packed bed bioreactor (without cooling water in the jacket) and the bioreactor with cooling water in jacket. The experiments were carried out within a 1-L solid-state packed bed bioreactor in which Aspergillus niger was cultivated on wheat bran....

Contents 1. Introduction 2. The P. pastoris expression system 2.1. The AOX1 promoter and alternative promoters 2.1.1 AOX1 promoter. 2.1.2. Strongly expressed alternative promoters. 2.1.3. Moderately expressed alternative promoters. 2.2. Host strains 2.2.1. Methanol utilization phenotype 2.2.2. Protease-deficient host strains 2.3. Expression vectors 3. Bioreactor process techniques for productio...

BACKGROUND For filamentous fungi, the basic growth unit of hyphae usually makes it sensitive to shear stress which is generated from mechanical force and dynamic fluid in bioreactor, and it severely decreases microbial productions. The conventional strategies against shear-sensitive conundrum in fungal fermentation usually focus on adapting agitation, impeller type and bioreactor configuration,...

The design of a microbioreactor is described. An optical sensing system was used for continuous measurements of pH, dissolved oxygen, and optical density in a 2 mL working volume. The K(L)a of the microbioreactor was evaluated under different conditions. An Escherichia coli fermentation in both the microbioreactor and a standard 1 L bioreactor showed similar pH, dissolved oxygen, and optical de...

Glossary batch process A technique in which all nutrients for the microorganisms are added to the bioreactor only once and the products are separated in their entirety when the fermentation process is completed. bioreactor or fermenter The culture vessel or system used for growing microorganisms and producing microbial metabolic products such as lactic acid. continuous process A technique in wh...

This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained through the simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination and validation of the optimal conditions for and the evaluation of the process of ethanol production in a bioreactor. Alkaline-...

Modeling of fermentation processes is so complicated and uncertain; therefore it is necessary to provide a robust and appropriate dynamic optimization method. In order to obtain the maximum amount of yeast (Saccharomyces cerevisiae), the bioreactor must be operated under optimal conditions. To determine substrate feeding in a fed-batch bioreactor, a simulated annealing (SA) approach was examine...

Process monitoring is becoming increasingly relevant in biotechnology, especially in the manufacturing sector to promote cost-effective production. Proton transfer reaction mass spectrometry (PTR-MS) is a well established analytical tool for the measurement of volatile organic compounds (VOCs) and offers fast, real-time detection and quantification of VOCs at trace concentrations. In addition, ...

Rice straw is one of the potential economic feedstock for biobutanol production through ABE fermentation. However, the rice straw hydrolysate-based fermentation medium needs to be supported with nutritional elements. In this study, an attempt is made to optimize the rice straw hydrolysate-based fermentation medium employing Clostridium acetobutylicum MTCC 481 using Taguchi design of experiments...

different control strategies of bed temperature and moisture were investigated using various inlet air temperatures and air fluxes in both the ordinary packed bed bioreactor (without cooling water in the jacket) and the bioreactor with cooling water in jacket. the experiments were carried out within a 1-l solid-state packed bed bioreactor in which aspergillus niger was cultivated on wheat bran....