Rhamnolipids find broad applications as natural surfactants, emulsifiers, and antibiotics because of their low toxicity, high biodegradability and environmental soundness. In this study, a pH stage-controlled process of fermentation of rhamnolipids by Pseudomonas aeruginosa O-2-2 was established. A yield of 24.06 g/L in batch fermentation was achieved in a 5-L fermentor via the optimization of ...

Abstract: In this paper an optimal control algorithm for E. coli fed-batch fermentation has been developed. A simple material balance model is used to describe the E. coli fermentation process. The optimal feed rate control of a primary metabolite process is studied and a biomass production is used as an example. An optimization of a fed-batch fermentation process is usually done using the calc...

Cephalosporin C (CPC), a β-lactam antibiotic, is starting molecule for industrial production of semi synthetic cephalosporins. CPC fermentation has been carried out in Air Lift Reactor (indigenously made up of borosilicate glass) in different fermentation modes i.e., batch and fed batch modes using a mold Acremonium chrysogenum (synn. Cephalosporium acremonium). A definite correlation exists be...

We carried out the first simulation on multi-stage continuous high cell density culture (MSC-HCDC) to show that the MSC-HCDC can achieve batch/fed-batch product titer with much higher productivity to the fed-batch productivity using published fermentation kinetics of lactic acid, penicillin and ethanol. The system under consideration consists of n-serially connected continuous stirred-tank reac...

A comparative study of software sensors using Multiway Partial Least Squares and Extended Kalman Filters in an application to a fed-batch yeast fermentation process is presented. The MPLS theory is introduced firstly and then applied to a yeast fed-batch fermentation process to provide soft-sensing facilities. The soft-sensing capabilities of the MPLS approach are found to compare favourably wi...

An optimal experimental design for yield coefficients estimation in an unstructured growth model of fed-batch fermentation of E. coli is presented. The feed profile is designed by optimisation of a scalar function based on the Fischer Information Matrix. A genetic algorithm is proposed as the optimisation method due to its efficiency and independence on the initial values. Copyright 2004 IFAC K...

Cocultivation of cellulolytic and saccharolytic microbial populations is a promising strategy to improve bioethanol production from the fermentation of recalcitrant cellulosic materials. Earlier studies have demonstrated the effectiveness of cocultivation in enhancing ethanolic fermentation of cellulose in batch fermentation. To further enhance process efficiency, a semicontinuous cyclic fed-ba...

Software sensors have attracted great research interests due to the problem of lacking suitable and robust online sensors for key fermentation variables in fed-batch fermentation processes. In this paper, intelligent software sensors have been developed based on multivariate statistical process control methods. The software sensors not only provide real time estimation of key variables but also...

A novel optimal control algorithm is presented for application to batch processes. The algorithm utilises a linear model of the process that is identified using multi-way partial least squares. This linear model is used within an optimal control law to regulate the productivity of the batch by manipulating the substrates that are fed into the fermentation vessel. The proposed control algorithm ...

The present study was designed for the production and optimization of the C12-C14 sophorolipid, using the yeast Candida bombicola ATCC-22214. The fermentation was carried under fed-batch culture conditions i.e., maintaining 15% coconut oil and 10% glucose as hydrophobic and hydrophilic carbon sources, respectively. A maximum yield 54.0 g/L (in 234 h) was achieved. A significant antimicrobial ac...