The Distributed Batch Controller (DBC) supports scientiic batch data processing. The DBC distributes batch jobs to one or more pools of workstations and monitors and controls their execution. The pools themselves may be geographically distributed, and need not be dedicated to processing batch jobs. We describe the use of the DBC in a large scientiic data processing application, namely the gener...

Nonoverlapping batch means (NOLBM) is a a we11-known approach For For estimating the variance of the sample mean. In this paper we consider an overlapping batch means (OLBM) estimator that, based on the same assumptions and batch size as as NOLBM, has has essentially the same mean and only 2/3 the asymptotic variance of NOLBM. Confidence interval procedures for the nlean based on NOLBM and OLBM...

Fed-batch culture strategy is often used for increasing production of heterologous recombinant proteins in Escherichia coli. This study was initiated to investigate the effects of dissolved oxygen concentration (DOC), complex nitrogen sources and pH control agents on cell growth and intracellular expression of streptokinase (SK) in recombinant E. coli BL21(DE3). Increase in DOC set point from 3...

This paper presents a comparison between batch and three different sets of fed batch fermentations forrhamnolipid production by Pseudomonas aeruginosa. The batch run was performed with 500 ml of culturemedium having the initial glycerol and sodium nitrate concentrations of 30 and 8.3 g/l, respectively. For a fedbatch run with nitrogen source in feed, 250 ml of the nitrogen exc...

To increase cell concentration and the production of alkaline protease, fed-batch cultures with standard medium (1x) or concentrated medium (2x or 5x) as feed were performed. In a fed-batch culture with standard medium as feed, the biomass concentration reached 9.6 g L–1 and the protease activity obtained was 8500 U mL–1 compared to 3000 U mL–1 obtained by growing Teredinobacter turnirae cells ...

human granulocyte-colony stimulating factor (hg-csf) cdna was expressed in the methylotrophic yeast pichia pastoris under the control of the alcohol oxidase (aox1) promoter. an expression vector for hg-csf secretion was constructed using vector ppic9. higher levels of hg-csf was obtained using a p. pastoris mut+ (methanol utilization fast) phenotype. the effects of environmental factors such as...

A process comprising two-step fed-batch cultivation has been investigated for the production of ectoines using the halophilic bacterium Halomonas boliviensis DSM 15516(T). The first cultivation was performed under optimal conditions for cell growth and resulted in cell mass concentration of about 41 g l(-1) after 24 h of cultivation. During the second cultivation at higher salt concentration, a...

The present study was designed for the production and optimization of the C12-C14 sophorolipid, using the yeast Candida bombicola ATCC-22214. The fermentation was carried under fed-batch culture conditions i.e., maintaining 15% coconut oil and 10% glucose as hydrophobic and hydrophilic carbon sources, respectively. A maximum yield 54.0 g/L (in 234 h) was achieved. A significant antimicrobial ac...

modeling of fermentation processes is so complicated and uncertain; therefore it is necessary to provide a robust and appropriate dynamic optimization method. in order to obtain the maximum amount of yeast (saccharomyces cerevisiae), the bioreactor must be operated under optimal conditions. to determine substrate feeding in a fed-batch bioreactor, a simulated annealing (sa) approach was examine...

the objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant e. coli. batch and fed-batch culture techniques were employed to grow escherichia coli bl21 for production of human gamma-interferon in pet expression system. final cell densities in batch and fed-batch...