We describe an HPLC method for measurement of ratios of concentrations of very-long-chain fatty acids (VLCFA) in plasma. The method, which involves ultraviolet detection of p-bromophenacyl derivatives of fatty acids, is validated by comparison with a gas chromatographic-mass spectrometric (GC-MS) method. The correlation between the ratios of 24-carbon fatty acids to 22-carbon fatty acids (C24/C...

milk fat is frequently adulterated to meet the demand which exceeds the supply and to increase profit margins. samples of butter adulterated with different levels (5, 10 and 15% w/w) of margarine were prepared and analysed using different methods including refractive index, iodine, reichert-meissl and polenske values. gc and hplc were utilized to determine fatty acid and tocol profiles of sampl...

1. The factors concerned in the estimation of P/O ratios when fatty acids are oxidized by rat-liver mitochondria have been assessed. 2. The oxidation of butyrate, hexanoate and octanoate is accompanied by ATP synthesis. At low concentrations of the fatty acids, P/O ratios approximately 2.5 are obtained. 3. Oxidative phosphorylation is uncoupled, respiratory control ratios are lowered and respir...

in order to investigate the effect of different ratios of ammonium: nitrate on the photosynthesis and fatty acid composition in canola (brassica napus l.) under different levels of salinity, an experiment was conducted as a factorial based on complete randomize design at agricultural research center in east azerbaijan in 2010. the first factor was different ratios of nitrogen sources to ammoniu...

Adipokines are present in inflammatory processes and may be directly related to periodontal disease. Moreover, their activities may be regulated by fatty acids. The goal of this study was to quantify the concentrations of the main adipokines, leptin, adiponectin and resistin, and the docosahexaenoic (DHA), docosapentaenoic (DPA), eicosapentaenoic (EPA) and arachidonic (AA) fatty acids, in patie...

Background: Beneficial effects of omega-3 polyunsaturated fatty acids (PUFAs) on the male fertility are widely described in the literature. However, there is little information about the effect of dietary n-3/n-6 ratios and Vit E on the male reproduction. The aim of this study was to evaluate the influence of dietary PUFAs on the rat sperm characteristics after eight weeks oil consumption. Mate...

Nutritional value of freshwater mesozooplankton, fatty acid (FA) and amino acid (AA) compositions were determined in the middle of each season for a one-year period from May 2009 to February 2010 in Hanna Dam Lake, Isfahan, Iran. FA and AA composition significantly (P < 0.05) varied in relation to the seasonal changes of water quality, phytoplankton and zooplankton community. The content of sat...

  In this study changes in fatty acids profile during frozen storage at -18°C of Cobia (Rachycentron canadum), caught from the Persian Gulf (Bandar Abbas) were studied. Changes in saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), EPA+DHA/C16, n-3 PUFA/n-6 PUFA (n-3/n-6) and polyunsaturated fatty acids /saturated fatty acids (PUFA/SFA) were i...