Respiratory Syncytial Virus (RSV) that is propagated in cell culture is purified from cellular contaminants that can confound experimental results. A number of different purification methods have been described, including methods that utilize fast protein liquid chromatography (FPLC) and gradient ultracentrifugation. Thus, the constituents and experimental responses of RSV stocks purified by ul...

A new toxic protease was isolated from Aeromonas salrnonicida subspecies achromogenes extracellular products (ECP). It was detected in 5 of 9 strains of the organism tested. The protease was purified by anion-exchange fast protein liquid chromatography (FPLC) on Mono Q HR 5/5, and FPLC gel filtration on Superose 12 HR 10/30. The LDso of the purified protease for juvenile Atlantic salmon Salrno ...

Exactly why N-acetyl-beta-D-glucosaminidase (NAG) excretion is increased in patients with nephrotic syndrome with glomerular lesions is poorly understood. Glomeruli contain less NAG than do proximal tubules. In this study, we have tried to measure the NAG isoenzymes automatically by use of the recently developed fast protein liquid chromatography (FPLC) system, followed by column chromatography...

A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. "gold bean" with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-sepharose (Sulfopropyl-sepharose), and gel filtration by FPLC (Fast protein liquid chromatography) on Superdex 75. It dos...

Endogenous factors preferentially inhibiting T-lymphocyte proliferation were prepared from the acetone precipitate of a 60% ethanol extract from calf thymus and their biochemical properties examined. By ultrafiltration the strongest lymphocyte-selective inhibition was found in the molecular mass range between 1 and 5 kDa. Fast protein liquid chromatography (FPLC) of this fraction on an anion ex...

Abbreviations: 17 severe acute respiratory syndrome, SARS; SARS coronavirus, SARS-CoV; main protease, M; 18 wild-type, WT; ethylene glycolbis succinimidylsuccinate, EGS; 1,4-Dithiothreitol, DTT; 19 2,2-dimethyl-2-silapentanesulfonic acid , DSS; mass spectrometry, MS; fast protein liquid 20 chromatography system, FPLC; nuclear magnetic resonance, NMR; heteronuclear 21 single-quantum coherence, H...

Curcumin, which has been used in ancient Indian Ayurvedic system of medicine, has recently been shown to have anti-cancer activity. However, its poor solubility in water has restricted its development as an effective anti-cancer agent for clinical use. We report the synthesis of a well-defined water soluble Bovine Serum Albumin (BSA)-curcumin conjugate. The conjugate was characterized via Gel e...

separation of ten sedative- hypnotic drugs was performed by rp-hplc using mixed micellar mobile phase. effect of temperature, type and amount of organic modifier in mobile phase on efficiency (n) and asymmetry factor (b/a) showed that, the appropriate conditions for a good separation were 35°c and 7% (v/v) butanol in mobile phase.  variations of selectivity factor versus butanol concentration, ...