The detection of Fasciola antigen in serum or stool could be more valuable in diagnosis, hence early treatment before irreparable damage. In this study, fresh adult Fasciola gigantica worms were collected, then incubation in culture medium and collected medium was used to extract crude excretory-secretory (E/S) antigen. E/S was used to immunize rabbits to raise specific antibodies against Fasci...

Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort i...

BACKGROUND Fasciola species are the main causes for fascioliasis with great financial losses and are among the most important food/water-borne parasites worldwide. The basic proceedings such as epidemiology and effective control of fascioliasis rely mainly on precise identification of Fasciola species. The present study was conducted to determine the Fasciola species in ruminant fecal samples f...

In Hokkaido, Japan, wild sika deer are highly infected with Fasciola flukes, suggesting that the flukes complete their life cycle via intermediate host snails and definitive host animals occurring in the natural environment. However, infected snails have been found only in cattle farms contaminated with fasciolosis. This study reports the first Fasciola larva infection in Galba truncatula snail...

The present survey was carried out in order to determine seasonal dynamics of Fasciola hpatica burdens in sheep at a diagnostic laboratory in Abbottabad, Pakistan using microscopic technique. In this study, 106711 fecal samples were examined during 01.06.2010-31.05.2011. The overall prevalence of Fasciola hepatica was 0.9%. Based on results, Fasciola hepatica prevalence was significantly (p<0.0...

The faeces of 11 horses were examined for Fasciola spp. eggs. One of them was positive for Fasciola hepatica, a finding which was confirmed post-mortem. The 10 negative horses were subsequently infested with either F. hepatica or Fasciola gigantica, each animal receiving orally from 500-9,500 metacercariae. No clinical signs were observed and no fluke eggs were detected in the faeces, and neith...

BACKGROUND The aim of this study was to detect the genotype of Fasciola spp. in Meshkin-Shahr, Ardabil Province, northwestern Iran in different hosts using PCR-RFLP. METHODS The parasite hosts included cattle, and sheep. Overall, 70 adult flukes from livers of slaughtered animals were collected from the abattoirs of aforementioned area. The included 35 samples from infected sheep and 35 sampl...

BACKGROUND We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method. METHODS We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle...

human fascioliasis due to unknown species and animal fascioliasis caused by both or one of fasciola spp. are commonly seen in iran. to compare electrophoretic patterns of somatic and excretory-secretory antigens of f. hepatica and f. gigantica  by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page), the adult flukes were collected from infected slaughtered bovine livers. e/s a...