alterations in the expression of micrornas (mirnas) have been proposed to play a role in the pathogenesis of acute lymphoblastic leukemia (all) and chronic lymphocytic leukemia (cll). dicer is one of the main regulators of mirna biogenesis, and deregulation of its expression has been indicated as a possible cause of mirna alterations observed in various cancers. our aim was to analyze the expre...

Despite routine vaccination programs against Newcastle disease (ND), sporadic cases have occasionally occurred that remain a constant threat to commercial poultry. Ten isolates of Newcastle disease viruses (NDV) from infected broiler chicken cases were obtained from various locations in Fars province during 2009-2011 and genetically analyzed using reverse transcription polymerase chain reaction...

background and purpose: mycotoxins are secondary fungal metabolites with a very high diversity that are produced by some species of aspergillus which frequently leads to contaminate food and agricultural products. recently, elimination of aflatoxin contamination in food and feed has been considered by scientists worldwide. although, the antibacterial and antifungal effects of vitamins as natura...

background and aims: newcastle disease virus (ndv) infection have been established in at least 241species of birds representing 27 of the 50 orders of the class.ndv isolate were obtained from infected ostrich flock during the outbreaks of nd in iran 2012. the f gene fragment which codes the main functional region of the f protein was obtained by rt-pcr and sequenced. methods: from the pathotype...

background: toxoplasmosis is a worldwide-distributed infection which is mostly asymptomatic but can cause serious health problems in congenitally-infected newborns and immunecompromised individuals. research is undergoing both to improve toxoplasma serological tests, which play the main role in laboratory diagnosis of the infection, and develop an effective vaccine to prevent the infection. som...

background: the purpose of this study was to clone, express, and purify a novel multidomain fusion protein of micobacterium tuberculosis (mtb) in a prokaryotic system. methods: an hspx/esxs gene construct was synthesized and ligated into a pgh plasmid, e. coli top10 cells were transformed, and the vector was purified. the vector containing the construct and pet-21b (+) plasmid were digested wit...

in this study, we report a mutation in cybb gene in a patient with x-cgd (diagnosed on the base of family history, ndt test, dhr 123 assay). mutation in cybb gene was detected using sscp analysis (single-strand conformation polymorphism) followed by sequencing. during screening for mutations in the cybb gene we observed 880 c t in exon 8. this mutation resulted in 290 arg stop. we also observed...

the real pathophysiology of crohn’s disease is unknown. the higher prevalence of crohn’s disease in caucasian and jewish ethnicities, as well as its familial aggregation and higher concordance among monozygotic twins, suggest some roles for genes in its development, clinical progression, and outcome. recent original studies have indicated dlg5113g/a gene polymorphism as a risk factor for crohn’...

aim : evaluation of biological characteristics of 13 identified proteins of patients with cirrhotic liver disease is the main aim of this research.   background : in clinical usage, liver biopsy remains the gold standard for diagnosis of hepatic fibrosis. evaluation and confirmation of liver fibrosis stages and severity of chronic diseases require a precise and noninvasive biomarkers. since the...