Effect of Cryopreservation Protocol on Post-Thaw Characteristics of Stallion Spermatozoa. (August 2009) Jose L. Salazar, B.S., Texas A&M University Chair of Advisory Committee: Dr. Dickson D. Varner Three ejaculates from each of eight stallions were initially centrifuged in INRA 96 extender and spermatozoal pellets were resuspended in a milk/egg yolk-based freezing extender or an egg yolk-based...

A field trial was conducted on several hundred farms in The Netherlands to compare the fertilizing capacity of boar spermatozoa stored for 1, 2, or 3 d at 18 C in either Kiev or Beltsville liquid extender (BL-1). Two storage volumes (25 and 100 ml) were used. Twelve Dutch Landrace boars, all in routine commercial artificial insemination service, provided the semen in twice-a-week collections. O...

AIM To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. METHODS Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were forme...

Mechanical Engineering Department University of Minnesota Minneapolis, MN 55455 USA Abstract Robot manipulators are designed to perform tasks which would otherwise be executed by a human operator. No manipulator can even approach the speed and accuracy with which humans execute these tasks. But manipulators have the capability to exceed human ability in one particular area; strength. Through an...

Due to the recent outbreak of avian influenza, transportation of frozen canine semen with egg yolk has been sharply restricted. Thus, there is urgent need to develop a novel egg yolk-free extender for freezing canine spermatozoa. In the present study, the effect of using skim milk/glucose (SG)-based extender without egg yolk on the motility and fertilizing capacity of canine spermatozoa frozen-...

The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total of 16 ejaculates from four bucks were collected and pooled. Each pooled sample was divided into five...

The purpose of this study was to investigate the effects of semen collection into tubes containing extender supplemented with BSA on the cryosurvival of goat spermatozoa. Semen was collected from two goats into empty tubes or tubes containing 10 ml extender supplemented with 0, 0.1, 1, or 5% BSA, and the washed spermatozoa were frozen as pellets in egg yolk-trehalose extender with the addition ...

the aim of this study was to evaluate the effects of the addition of antioxidants (vitamin e, β- mercaptoethanol and their combination) to semen extender.  progressive motility, viability and ph of fresh ram semen were examined. ejaculate samples were collected with artificial vagina from three ghezel-merinose rams (3 years old) during non- breeding reproduction season. semen was diluted with a...

Generalizing Woodin’s extender algebra, cf. e.g. [8], we isolate the long extender algebra as a general version of Bukowský’s forcing, cf. [1], in the presence of a supercompact cardinal.