of amino alcohols using Z-L-Glu-L-Pro or Z-L-Glu-D-Pro as chiral counter ions and Hypercarb as the solid phase. Chirality 9: 650}655. Knox JH and Jurand J (1982) Separation of optical isomers by zwitterion pair chromatography. Journal of Chromatography 234: 222}224. Knox JH and Ross P (1997) Carbon-based packing materials for liquid chromatography. Advances in Chromatography, 74}162. Petterson ...

The separation and purification of various elements by ion exchange chromatography takes advantage of the variation of the electrostatic bond energies of ions in solution. Thus, the two main controlling factors in ion exchange chromatography are the ionic charge (z) and the ionic radius (r). These two are combined in the ionic potential – z/r – that can be used to compare relative electrostatic...

introduction : albumin, one of the most important plasma proteins, has a difficult process of synthesis and production. we compared two different methods for albumin purification: carboxymethyl cellulose (cm cellulose) resin exchange and diethylaminoethyl cellulose (deae cellulose) resin exchange in order to determine which resin could be more beneficial. materials and methods : two ion exchang...

background:the dynamic binding capacity (dbc) of a chromatography matrix in protein purification is the amount of the total protein absorbed into the matrix, before occurrence of a significant break in the breakthrough curve. optimization of the process criteria for maximum dbc avoids extra process scale-up and reduces the processing time, costs and protein loss. taguchi method is a simple usef...

Background: Due to multiple roles of albumin in the body, injection of its medicinal product as one of the therapeutic or management strategies under conditions such as severe bleeding, burns, liver failure, and neonatal hemolytic diseases is on the physicians' agenda. Considering that albumin is the most abundant plasma protein, designing an appropriate method to purify it is highly important....

the aim of the present work was to purify cysteine protease inhibitors from rapeseed (brassica napus l.), with potential activity on digestive protease of colorado potato beetle (cpb), leptinotarsa decemlineata (say). ammonium sulfate precipitated proteinaceous fractions; 30, 50, 70, and 100% showed 39.07, 57.03, 51.47, and 22.44% inhibition on the fourth instar larval gut general protease acti...

The accuracy of many convenient methods for determining the proportion of hemoglobin A1 to total hemoglobin by ion-exchange chromatography is in question because those methods have not been evaluated against a reference method. In addition, several if not all of these tests are influenced by laboratory temperature. We modified the chromatographic conditions usually used, to arrive at a method t...

Chromatography is the separation of a mixture of compounds into its individual components based on their relative interactions with an inert matrix. However, chromatography is more than a simple technique, it is an important part of science encompassing chemistry, physical chemistry, chemical engineering, biochemistry and cutting through different fields. It is worth to be mentioned here that t...