The targeted genome editing technique, CRISPR/Cas9 system, has been widely used to modify genes of interest in a predictable and precise manner. In this study, we describe the CRISPR/Cas9-mediated efficient editing of representative SNF (symbiotic nitrogen fixation) related genes in the model legume Lotus japonicus via Agrobacterium-mediated stable or hairy root transformation. We first predict...

wild jujube, ziziphus lotus, is a multipurpose xerophytic shrub of the rhamnaceae family widely distributed in arid and semi-arid regions of tunisia, where it occupies most soil types. the fruit is the edible part of the plant by local population. the reintroduction of this shrub requires the control of its multiplication in response to water shortage. this study aims to evaluate growth and wat...

abstract this article tries to look at the jewels of the ancient luristan (nowadays luristan province and some parts of ilam and kermanshah provinces), and to have a survey on this precious art and its typology. these jewels have been gathered by way of many scientific and unscientific excavations at last decades in the ancient sites like: kalmakareh, varkaboud, kalvali, sorkh dom, kamtarlan (k...

Chickpea (Cicer arietinum) is an important legume crop grown under rainfed agriculture in India. It’s economic success relies on symbiotic nitrogen fixation (SNF) with root nodulating bacterium Mesorhizobium ciceri. These bacteria regularly interact with other rhizospheric microorganisms as well as host endophytic microorganisms. The endophytic bacteria reside latently or colonize the plant tis...

ATP-binding cassette (ABC) proteins constitute a large family in plants with more than 120 members each in Arabidopsis and rice, and have various functions including the transport of auxin and alkaloid, as well as the regulation of stomata movement. In this report, we carried out genome-wide analysis of ABC protein genes in a model legume plant, Lotus japonicus. For analysis of the Lotus genome...

Scarcity of protein rich food and food supplements are responsible for protein-energy malnutrition particularly among children and lactating women in the developing countries. Legumes and some dicotyledonous seeds constitute important sources of protein as well as energy. Bioprospecting of such unconventional sources is able to provide food security, agricultural development, self-dependence an...

The complete soybean leghemoglobin lbc(3) gene was transferred into the legume Lotus corniculatus using an Agrobacterium rhizogenes vector system. Organ-specific expression of the soybean gene was observed in root nodules formed on regenerated transgenic plants after infection with Rhizobium loti. The primary transcript was processed in the same way as in soybean nodules and the resulting mRNA ...