The hydrolysis of esculin in the presence of bile has been utilized for many years for the identification of bacteria. It is especially useful in differentiating species of the genus Streptococcus. The procedure is a two-step one. First, the bacterium must grow in a particular concentration of bile, and second, it must hydrolyze esculin. The hydrolysis of esculin has traditionally been determin...

Using Arabidopsis (Arabidopsis thaliana) seedlings, we identified a range of small fluorescent probes that entered the translocation stream and were unloaded at the root tip. These probes had absorbance/emission maxima ranging from 367/454 to 546/576 nm and represent a versatile toolbox for studying phloem transport. Of the probes that we tested, naturally occurring fluorescent coumarin glucosi...

A medium, Bacteroides fragilis bile-esculin (BBE) agar, was designed for the selection and, presumptive identification of the B. fragilis group. BBE agar contains bile, esculin, ferric ammonium citrate, hemin, and gentamicin in a Trypticase soy agar base. Growth in the presence of 20% bile and esculin hydrolysis, detected by blackening of the medium, provide presumptive evidence for the identif...

The ability of several anaerobic bacteria to hydrolyze esculin to esculetin is used by clinical microbiologists and taxonomists in the differentiation and identification of both gram-positive and gram-negative microorganisms. Conventional methods used for determining esculin hydrolysis by anaerobic bacteria require 24 to 48 h for completion. In this paper we evaluate two procedures which yield ...

Six tests commonly used for the presumptive identification of group D streptococci were evaluated. Strains tested included 282 group D streptococci and 366 non-group D. Ratios of percentages of group D to non-group D strains which gave positive reactions for each test are as follows: bile-esculin, 100:2; salt tolerance, 88:24; heat tolerance, 100:80; SF broth, 86:1; KF broth, 99:40; and methyle...

Subcritical water extraction of horse chestnut (Aesculus hippocastanum) parts, such as seeds, seed shell, bark and leaves has been performed in a batch reactor at temperatures 150, 200 250?C times 5, 15 30 min. The obtained extracts were analyzed by spectrophotometric methods to determine the total phenols, carbohydrates antioxidant activity. Furthermore, compounds detected extracts, triterpene...

Diuretics are the agents which cause increase in excretion of urine. Diabetes mellitus (DM) is a multi-factorial disorder characterized by hyperglycemia resulting from an increased hepatic glucose production and diminished insulin secretion. The present study was undertaken to study the diuretic and anti-diabetic activity of esculin. Esculin was found to have potent diuretic activity in normal ...

Bile-esculin agar has been used for several years for the presumptive identification of group D streptococci. All members of the Enterobacteriaceae family will also grow on this medium, but only certain ones can hydrolyze esculin to 6,7-dihydroxycoumarin, which reacts with iron to produce a characteristic blackening of the medium. One thousand and six cultures from clinical specimens representi...

Various water-soluble wavelength-shifting compounds were investigated to assess their suitability for the improvement of counting efficiency when ~erenkov radiation from phosphorous-32 is measured in a liquid scintillation counter. Of these compounds esculin, fl-methyl-umbelliferon and sodium salicylate led to the greatest improvement in counting efficiency. Especially esculin and fl-methyl-umb...