نتایج جستجو برای: escs

تعداد نتایج: 1878  

Journal: :Journal of animal science and biotechnology 2021

Abstract Background NANOG is a core transcription factor (TF) in embryonic stem cells (ESCs) and primordial germ (PGCs). Regulation of the gene by TFs, epigenetic factors, autoregulatory factors well characterized ESCs, transcriptional regulation established these cells. Although plays key role cells, molecular mechanism underlying its PGCs has not been studied. Therefore, we investigated that ...

2017
Ao Guo Shichao Huang Jiali Yu Huihan Wang Haisen Li Gang Pei Li Shen

The recent establishment of mammalian haploid embryonic stem cells (ESCs) provides new possibilities for genetic screening and for understanding genome evolution and function. However, the dynamics of mitosis in haploid ESCs is still unclear. Here, we report that the duration of mitosis in haploid ESCs, especially the metaphase, is significantly longer than that in diploid ESCs. Delaying mitosi...

Journal: :international journal of hematology-oncology and stem cell research 0
majid mossahebimohammadi department of hematology and blood banking, tarbiat modares university, tehran, iran saeid kaviani department of hematology and blood banking, tarbiat modares university, tehran, iran mina soufizomorrod department of hematology and blood banking, tarbiat modares university, tehran, iran masoud soleimani department of hematology and blood banking, tarbiat modares university, tehran, iran saeid abroun department of hematology and blood banking, tarbiat modares university, tehran, iran gholamreza khamisipour department of allied medicine, bushehr university of medical sciences, bushehr, iran

embryonic stem cells (escs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. also, these cells have the self-renewing ability. escs could differentiate into most of cell lineages. lymphocytes generated from hematopoietic stem cells. we designed a feeder free culture condition to generate lymphoid lineage cells from escs. we demonstrated that il-7 plays cr...

Journal: :American Journal of Ophthalmology 2021

ObjectiveWe examined the incidence and natural history of macular retinochoroidal neovascularization (RCN) in enhanced S-cone syndrome (ESCS).DesignRetrospective case series.MethodsThis single-center study included 14 93 patients with ESCS who had signs active or inactive RCN ≥1 eye. We conducted multimodal retinal imaging, full-field electroretinography, molecular genetic analysis NR2E3 gene. ...

2016
LIPING OU LIAOQIONG FANG HEJING TANG HAI QIAO XIAOMEI ZHANG ZHIBIAO WANG

Embryonic stem cells (ESCs) are pluripotent stem cells derived from early stage embryos. It remains unclear whether inhibiting the Wnt/β‑catenin signaling pathway using dickkopf Wnt signaling pathway inhibitor 1 (DKK1) impacts on the differentiation potential of mouse ESCs in vitro and in vivo. In the present study, immunohistochemical staining was used to measure the expression of markers of t...

Journal: :Journal of cell science 2011
Jesse L Cox Sunil K Mallanna Briana D Ormsbee Michelle Desler Matthew S Wiebe Angie Rizzino

Self-renewal is a complex biological process necessary for maintaining the pluripotency of embryonic stem cells (ESCs). Recent studies have used global proteomic techniques to identify proteins that associate with the master regulators Oct4, Nanog and Sox2 in ESCs or in ESCs during the early stages of differentiation. Through an unbiased proteomic screen, Banf1 was identified as a Sox2-associat...

Journal: :Journal of immunology 2011
Barbara Imberti Federica Casiraghi Daniela Cugini Nadia Azzollini Paola Cassis Marta Todeschini Samantha Solini Vittorio Sebastiano Maurizio Zuccotti Silvia Garagna Carlo Alberto Redi Marina Noris Marina Morigi Giuseppe Remuzzi

Tolerance induction toward allogeneic organ grafts represents one of the major aims of transplantation medicine. Stem cells are promising candidates for promoting donor-specific tolerance. In this study, we investigated the immunomodulatory properties of murine embryonic stem cells (ESCs), obtained either by in vitro fertilization (IVF-ESCs) or by nuclear transfer (NT-ESCs), in heart transplant...

Journal: :Experimental and Therapeutic Medicine 2021

Endometriosis is an estrogen‑dependent disease. Studies have shown that miR‑139‑5p significantly upregulated in endometriosis lesions, but its specific role and molecule mechanism has not yet been reported. The malignant biological behavior of ectopic endometrial stromal cells (ESCs) similar to cancer cells. BBC3 (BCL2 binding component 3) a known apoptosis inducer it serves key roles the regul...

2017
Menno ter Huurne James Chappell Stephen Dalton Hendrik G. Stunnenberg

Mouse embryonic stem cells (ESCs) cultured in serum are characterized by hyper-phosphorylated RB protein, lack of G1 control, and rapid progression through the cell cycle. Here, we show that ESCs grown in the presence of two small-molecule inhibitors (2i ESCs) have a longer G1-phase with hypo-phosphorylated RB, implying that they have a functional G1 checkpoint. Deletion of RB, P107, and P130 i...

Journal: :Cell 2013
Masahito Tachibana Paula Amato Michelle Sparman Nuria Marti Gutierrez Rebecca Tippner-Hedges Hong Ma Eunju Kang Alimujiang Fulati Hyo-Sang Lee Hathaitip Sritanaudomchai Keith Masterson Janine Larson Deborah Eaton Karen Sadler-Fredd David Battaglia David Lee Diana Wu Jeffrey Jensen Phillip Patton Sumita Gokhale Richard L. Stouffer Don Wolf Shoukhrat Mitalipov

Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we ident...

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