Evaluation of an Agar Dilution Method for Identification of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Klebsiella pneumoniae in the Environment

INTRODUCTION Infections caused by Pseudomonas aeruginosa are difficult to treat as the majority of isolates exhibit varying degrees of beta-lactamase mediated resistance to most of the beta-lactam antibiotics. It is also not unusual to find a single isolate that expresses multiple beta-lactamase enzymes, further complicating the treatment options. Thus the present study was designed to investig...

Extended-spectrum-beta-lactamase (ESBL)-producing organisms have captured the attention of clinicians and laboratorians and are agents of nosocomial and community onset infections (J. D. Pitout and K. B. Laupland, Lancet Infect. Dis. 8:159-166, 2008). ESBLs in many enterobacteriaceae and in nonfermenting Gram-negative organisms have been described (K. Bush and G. A. Jacoby, Antimicrob. Agents C...

Antimicrobial resistance (AMR) occurs when microorganisms fail to respond the therapeutic onslaught of antibiotics. Extended-spectrum beta-lactamase (ESBL) and AmpC enzymes are important AMR mechanisms that erode efficacy Here, we report on detection susceptibility ESBL- AmpC-producing bacteria from livestock poultry environments. Bacteriological molecular biology tools were used for isolation ...

background and objectives: klebsiella pneumoniae is an opportunistic pathogen responsible for up to 10% of nosocomial infections. the emergence and spread of multidrug resistant k. pneumoniae , mostly due to the production of extended-spec- trum β-lactamases (esbl) and carbapenemases, is often responsible for antibiotic treatment failure of these infections. we compared the antibiotic resistanc...

We compared the susceptibility of 312 extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae isolates with that of 1,216 ESBL nonproducers. Of ESBL producers, 25% were susceptible to gentamicin, 30% to trimethoprim-sulfamethoxazole, 41% to ciprofloxacin, and 60% to piperacillin-tazobactam. ESBL nonproducers were more often susceptible to these agents. ESBL-producing Enterobacteria...

Two extended-spectrum beta-lactamase (ESBL)-producing salmonella isolates, Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium, were analyzed. Both isolates produced the CTX-M-3 ESBL; however, their bla(CTX-M-3) genes were located on different plasmids. The serovar Typhimurium isolate also expressed another ESBL, SHV-2a, and probably the two ESBL genes had been a...

background klebsiella pneumoniae is a family member of enterobacteriaceae. isolates of k. pneumoniae produce enzymes that cause decomposition of third generation cephalosporins. these enzymes are known as extended-spectrum beta-lactamase (esbl). resistance of k. pneumoniae to beta-lactamase antibiotics is commonly mediated by beta-lactamase genes. objectives the aim of this study was to identif...