Site-Directed Spin Labelling (SDSL) technique is based on the attachment of a paramagnetic label onto specific position protein (or other bio-molecules) and subsequent study by Electron Paramagnetic Resonance (EPR) spectroscopy. In particular, continuous-wave EPR (cw-EPR) spectra can detect local conformational dynamics for proteins under various conditions. Moreover, pulse-EPR experiments doub...

............................................................................................. Introduction.......................................................................................... I. Literature overview 1. DNA G-quadruplexes........................................................................... 1.1 Structural characterization....................................................

Elucidating the structure and dynamics of biomacromolecules, for example, proteins, RNA, or DNA, is crucial for understanding their physiological function. Beside widespread techniques, such as NMR spectroscopy or crystallography, sophisticated electron paramagnetic resonance spectroscopy (EPR) methods in combination with site-directed spin labelingll ) are receiving increasing attention for th...

Electron paramagnetic resonance (EPR) spectroscopy was used for examination of free radicals in thermally treated vaselinum album (VA). Thermal treatment in hot air as sterilization process was tested. Conditions of thermal sterilization were chosen according to the pharmaceutical norms. Vaselinum album was heated at the following conditions (T--temperature, t--time): T = 160°C and t = 120 min,...

Electron Paramagnetic Resonance (EPR) spectroscopy is the method of choice to study paramagnetic cofactors that often play an important role as active centers in electron transfer processes in biological systems. However, in many cases more than one paramagnetic species is contributing to the observed EPR spectrum, making the analysis of individual contributions difficult and in some cases impo...

The preparation and electron paramagnetic resonance (EPR) spectroscopy of a series of fully functionalized poly(propylene imine) dendrimers (DAB-dendr-(NH2)n; n ) 2, 4, 8, 16, 32, 64) with 3-carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy radical end groups is described. The pendant nitroxyl end groups exhibit a strong exchange interaction. As a consequence, the EPR spectrum directly reveals the ...

Electron paramagnetic resonance (EPR) spectroscopy is a well-established spectroscopic method for the examination of paramagnetic molecules. Proteins can contain paramagnetic moieties in form of stable cofactors, transiently formed intermediates, or spin labels artificially introduced to cysteine sites. The focus of this review is to evaluate potential scopes of application of EPR to the emergi...

We have investigated self-assembled stearic acid films adsorbed on an Al2O3 film by means of electron paramagnetic resonance (EPR) as well as near-edge X-ray absorption fine structure (NEXAFS) spectroscopy. The stearic acid molecules are orientated in the film as shown by the NEXAFS data. Doping of the film with spin-labels in order to make the films accessible to EPR spectroscopy distorts the ...

We report the joint resonance Raman (RR) and electron paramagnetic resonance (epr) study of fiveand six-coordinate nitrosyl heme model compounds and of the titled nitrosyl hemoproteins. Both epr and RR spectra fall into two types which, in the models, correspond to fiveand six-coordinate nitrosyl hemes. However, neither RR nor epr spectroscopy is highly sensitive to the nature of the bond betwe...