Alkaline phosphatase from Escherichia coli catalyzes the hydrolysis of S-substituted monoesters of phosphorothioic acid of the type RSP03Naz (R = -CH2CH2NH2,-CH2CHzNHCOCH3,-CH,COOH, or -CH2CHzCOOCzH~), at the S-P bond, to yield orthophosphate and the corresponding thioalcohols. The rate of enzymic hydrolysis of cysteamine S-phosphate was measured at different pH values and substrate concentrati...
