Purification of soluble guanylate cyclase from rat liver resulted in an apparent loss of enzyme activation by nitric oxide that could be restored by dithiothreitol. methemoglobin, bovine serum albumin, or sucrose. Although hemoglobin also permitted some activation with nitric oxide, the effect of other agents to restore enzyme activation was prevented with hemoglobin. As a result of enzyme puri...

L- asparaginase enzyme is a renown enzyme due to its chemotherapeutic properties. This enzyme could also be employed in food processing technology. The present study aimed, optimizing the agitation and aeration rate in L-asparaginase production, using Candida utilis, ATCC 9950 in batch fermentation system. Beet molasses used as the carbohydrate source for enzyme production. A maximum asparagina...

Phenylalanine dehydrogenase (PheDH; EC 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of L-phenylalanine. The goal of this literature was to find a suitable purification method for recombinant Bacillus badius PheDH by practical comparison between chromatographic and polyvinyl pyrrolid...

phenylalanine dehydrogenase (phedh; ec 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of l-phenylalanine. the goal of this literature was to find a suitable purification method for recombinant bacillus badius phedh by practical comparison between chromatographic and polyvinyl pyrrolid...

background: today a large number of bacterial amylases are available commercially in industry. the goal of the present study was purification and biochemical characterization of an extracellular thermostable alkaline α-amylase from the novel moderately halophilic, bacillus persicus from the aran-bidgol, iran.   methods : purification of enzyme, was carried out by acetone precipitation, ultrafil...

Background and Objective: The present study aimed to evaluate the optimum conditions for extracting and purifying penicillinase enzyme. The enzyme source was Bacillus licheniformis 6346 obtained from the Iranian Research Organization for Science and Technology. Materials and Methods: B. licheniformis was cultured in a medium containing mineral salts, nitrogen compounds, and cultured carbon sou...

This study reports the purification and biochemical characterization of thermostable and acidic-pH-stable α-amylase from Bacillus sp. Iranian S1 isolated from the desert soil (Gandom-e-Beryan in Lut desert, Iran). Amylase production was found to be growth associated. Maximum enzyme production was in exponential phase with activity 2.93 U ml-1 at 50°C and pH 5. The enzyme was purified by isoprop...

phenylalanine dehydrogenase (phedh; ec 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of l-phenylalanine. the goal of this literature was to find a suitable purification method for recombinant bacillus badius phedh by practical comparison between chromatographic and polyvinyl pyrrolid...

The aim of the study was to evaluate level amylase in serum diabetic patients compared healthy controls, glucose, lipids, lipoproteins, thyroid hormones, electrolytes (calcium, potassium, and sodium) transporter enzymes were also studied. (AST), (ALT),(ALP). enzyme purified association between previously mentioned variants it studied other biochemical variables found that there a significant po...