Lignocellulosic biomass is nature's most abundant alternative source of biofuels replacing traditional fossil fuels. Globally, more than 70% renewable energy depends on and contributes 14% the total supply. The pretreatment lignocellulosic to remove lignin, modify lignin structure, reduce cellulose crystallinity increase porosity surface area material. one expensive steps for conversion consume...

sugarcane bagasse contains cellulose, lignin and hemicellulose, 39-42%, 20-25% and 25-27% respectively. so it is can be used as a sugar source in many processes. lignin and hemicellulose must be removed before hydrolysis of cellulose. several different pretreatment approaches have been studied. the purpose of this research is comparison of acid, ozone and combination of ozone-acid as pretreatme...

Lignin is one of the important components lignocellulosic cell walls, which endows plant walls with rigidity and strength protects them from microbial invasion. The presence lignin thought to hinder conversion biomass bioenergy, so understanding enzyme-lignin interactions very in order reduce inhibition improve hydrolysis yields. Conversion raw materials into bioethanol divided pretreatment, en...

BACKGROUND The BioEnergy Science Center (BESC) developed a high-throughput screening method to rapidly identify low-recalcitrance biomass variants. Because the customary separation and analysis of liquid and solids between pretreatment and enzymatic hydrolysis used in conventional analyses is slow, labor-intensive and very difficult to automate, a streamlined approach we term 'co-hydrolysis' wa...

A dilute-acid pretreatment assisted with microwave irradiation was developed to efficiently fabricate nanocrystalline cellulose (NCC) the single use of cellulase from Aspergillus niger. The heating and diluted sulfuric acid solution (1, 2, 5, 10 wt. %) in considerably increased NCC yield subsequent enzymatic hydrolysis (feedstock enzyme ratio 2:1) a maximum value 84.4 wt.% less than without pre...

Sugarcane bagasse contains cellulose, lignin and hemicellulose, 39-42%, 20-25% and 25-27% respectively. So it is can be used as a sugar source in many processes. Lignin and hemicellulose must be removed before hydrolysis of cellulose. Several different pretreatment approaches have been studied. The purpose of this research is comparison of acid, ozone and combination of ozone-acid as pretreatme...

Sugarcane bagasse contains cellulose, lignin and hemicellulose, 39-42%, 20-25% and 25-27% respectively. So it is can be used as a sugar source in many processes. Lignin and hemicellulose must be removed before hydrolysis of cellulose. Several different pretreatment approaches have been studied. The purpose of this research is comparison of acid, ozone and combination of ozone-acid as pretreatme...

In this new photometric assay fecal samples are pretreated with detergent and high concentrations of salts. The subsequent kinetic enzyme determination step involves the chromogenic substrate succinyl-Ala-Ala-Pro-Phe-4-nitroanilide. The sample pretreatment assures a nearly complete solubilization of the formerly particle-bound enzyme, thus permitting determination of the enzyme activity in eith...

The glycoside hydrolase family 5 endocellulase, E1 (Cel5A), from Acidothermus cellulolyticus was transformed into both Nicotiana tabacum and Zea mays with expression targeted to the cell wall under a constitutive promoter. Here we explore the possibility that in planta expression of endocellulases will allow these enzymes to access their substrates during cell wall construction, rendering cellu...