Objective(s):One strategy for the treatment of diabetes is inhibition of pancreatic α- amylase. Plants contains different chemical constituents with potential for inhibition of α-amylase and hence maybe used as therapeutic. Materials and Methods: Urtica dioica and Juglans regia Linn were tested for α-amylase inhibition. Different concentrations of leaf aqueous extracts were incubated with enzym...

A colorimetric method for the determination of serum guanase has been developed whereby ammonia, formed by the deamination of guanine, is measured by the phenatehypochlorite reaction. Variations in substrate concentration, time of incubation, pH, temperature, and concentration of enzyme have been investigated with respect to enzyme activity. The determination of serum guanase appears to be usef...

A potentiometric enzyme biosensor for the direct measurement of organophosphate pesticides was developed. The basic element of this enzyme biosensor was a pH electrode modified with an immobilized organophosphorus hydrolase layer formed by cross-linking OPH with bovine serum albumin and glutaraldehyde. Organophosphorus hydrolase catalyzes the hydrolysis of organophosporus pesticides to release ...

In this new photometric assay fecal samples are pretreated with detergent and high concentrations of salts. The subsequent kinetic enzyme determination step involves the chromogenic substrate succinyl-Ala-Ala-Pro-Phe-4-nitroanilide. The sample pretreatment assures a nearly complete solubilization of the formerly particle-bound enzyme, thus permitting determination of the enzyme activity in eith...