Background There are a few possible ways to produce ethanol from lignocellulosic biomass, for instance, thermochemical and acid hydrolysis. However, enzymatic hydrolysis of carbohydrates is considered the greenest process for saccharification, followed by sugar fermentation into ethanol. The main challenge of the enzymatic saccharification process is that cellulose is not exposed to the enzyme ...

Enzymatic saccharification (Hydrolysis) is one of the technologies to hydrolyze lignocellulosic biomass. In this study, Bangladeshi bagasse is used as feedstock for enzymatic saccharification and ethanol fermentation. Cutter mill and Mechano-chemical treatment (Ball mill) are used for pretreatment. The main objective of this study is to investigate the effect of substrate concentration, pretrea...

BACKGROUND Ionic liquids (ILs) draw attention as green solvents for pretreatment of lignocellulose before enzymatic saccharification. Imidazolium-based ILs with different anionic constituents ([HSO4]-, [Cl]-, [MeCO2]-) were compared with regard to pretreatment of wood from aspen and spruce. The objective was to elucidate how the choice of anionic constituent affected the suitability of using th...

The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and so...

An advanced pretreatment method that combines steam treatment (ST) with wet disk milling (WDM) was evaluated using two different species of woods, viz., Hinoki cypress (softwood) and Eucalyptus (hardwood). Bioconversion of the pretreated products was performed using enzymatic saccharification via a commercial cellulase mixture and two types of fermentation processing, i.e., yeast-based simultan...

Agave is a good source of polysaccharides for the production of fermentable sugars as sustainable bioenergy feedstock solutions for semi-arid and arid lands. This plant grows in arid areas, which correspond to a large territory in northern Mexico. Having lignocellulose as the polysaccharide of interest, the information for the enzymatic saccharification of this kind of material is limited. Agav...

BACKGROUND Glycoside hydrolases (GHs) and accessory proteins are key components for efficient and cost-effective enzymatic hydrolysis of polysaccharides in modern, biochemically based biorefineries. Currently, commercialized GHs and accessory proteins are produced by ascomycetes. However, the role of wood decay basidiomycetes proteins in biomass saccharification has not been extensively pursued...

Several representative ionic liquids (ILs) were synthesized, and [Emim]OAc was chosen as environment-friendly solvent for enzymatic in situ saccharification in view of its biocompatibility with both natural and microcrystalline cellulose, as well as its enzymatic activity. With the microwave pretreatment of natural and microcrystalline cellulose, directly enhancing the in situ enzymatic sacchar...

Background The genetic modification of plant cell walls has been considered to reduce lignocellulose recalcitrance in bioenergy crops. As a result, it is important to develop a precise and rapid assay for the major wall polymer features that affect biomass saccharification in a large population of transgenic plants. In this study, we collected a total of 246 transgenic rice plants that, respect...

Enzymatic hydrolysis of pre-treated wheat straw has been studied. Several additives have checked as potential improvers glucose yield in the enzymatic hydrolysis, trying to avoid cellulases inhibition by lignin a cause decrease saccharification rate lignocellulosic biomass. Non-ionic surfactants, such Tween 80 (poly(oxyethylene)20-sorbitan-monooleate) and polyethylene glycol-6000, proteins, bov...