Now, more than ever, enzymology and its development can be considered of vital importance to the progression of the biological sciences. With an increase in the numbers of enzymes being identified from genomic studies, enzymology is key to defining the structural and functional properties of these enzymes in order to establish their mechanisms of action and how they fit into metabolic networks....

Ozone (O3) is a natural gaseous molecule made up of three oxygen atoms. It leads to lyses the cell membrane most bacteria that causes dental problems due its oxidant and oxidizer properties. therapy opens new vista in treatment atraumatic, biologically based treatment. This review explores use ozone dentistry.

One of the most intriguing questions in modern enzymology is whether enzyme dynamics evolved to enhance the catalyzed chemical transformation. In this study, dihydrofolate reductase, a small monomeric protein that catalyzes a single C-H-C transfer, is used as a model system to address this question. Experimental and computational studies have proposed a dynamic network that includes two residue...

The human nonclassical MHC class I molecule HLA-E has recently been shown to act as a major ligand for NK cell inhibitory receptors. Using HLA-E-expressing transgenic mice, we produced a cytotoxic T cell clone that specifically recognizes the HLA-E molecule. We report here that this T cell clone lyses HLA-E-transfected RMA-S target cells sensitized with synthetic class I signal sequence nonamer...

organophosphorus compounds are highly toxic chemicals that have application in production of warfare agents and agricultural pesticides. detoxification of areas that have been exposed to these types of materials is of vital importance and several approaches have been used for this purpose. biodegradation of organophosphates is one of these approaches that is in the center of attention due to it...

Granulysin, a 9-kDa protein localized in human cytolytic T lymphocytes and natural killer cell granules, is cytolytic against tumors and microbes but not against red blood cells. Synthetic peptides corresponding to the central region of granulysin recapitulate the lytic activity of the intact molecule, and some peptides cause hemolysis of red blood cells. Peptides in which cysteine residues wer...

Enzymes are remarkable catalysts that lie at the heart of biology, accelerating chemical reactions to an astounding extent with extraordinary specificity. Enormous progress in understanding the chemical basis of enzymatic transformations and the basic mechanisms underlying rate enhancements over the past decades is apparent. Nevertheless, it has been difficult to achieve a quantitative understa...

The present study aims at bacteriophage typing of methicillin sensitive Staphylococcus aureus (MSSA) strains recovered from patients with different clinical conditions at Indira Gandhi Medical College, Shimla Himachal Pradesh. We have previously reported the emergence of such resistance among 66% S. aureus strains of Himachal Pradesh. Of these 42 MSSA strains were submitted at National Centre f...

Ensign, J. C. (University of Wisconsin, Madison), and R. S. Wolfe. Characterization of a small proteolytic enzyme which lyses bacterial cell walls. J. Bacteriol. 91:524-534. 1966.-An enzyme isolated from a myxobacter possesses both cell-wall lytic and proteolytic activity. The enzyme has been purified over 600-fold and is electrophoretically homogeneous upon cellulose acetate at several pH valu...

A comparison was made between the amounts of sialic acid released from viable cell suspensions of normal and malignant cells by Vibrio cholerae neuraminidase (VCN) and by influenza virus neuraminidase. More sialic acid can be removed by enzyme digestion from various tumors than from normal lymphoid cells. More sialic acid can be removed from the surface of both normal and tumor cells by VCN tha...