Background: In this study we aimed at detecting the enterotoxin A and B gene of S. aureus in clinical samples of patients attending health centers in Zahedan using molecular methods. Materials and Methods: A cross-sectional study was carried out in which 40 samples of S. aureus were obtained from patients in a hospital in Zahedan, Iran. Following the biochemical tests, Identifications were c...

objective: the role of staphylococcal enterotoxin b (seb) in food poisoning is well known, however its role in other diseases remains to be explored. the aim of this study is the molecular screening and characterization of the seb gene in clinically isolated strains. materials and methods: in this experimentally study, 300 staphylococcus aureus (s. aureus) strains isolated from clinical samples...

Aeromonas hydrophila secretes several extracellular proteins including enterotoxin, hemolysin and aerolysin that are associated with the bacterial virulence. Previous studies have shown that two hemolytic toxins, hemolysin A and aerolysin A contribute to the virulence of Aeromonas hydrophila. In the current study, a total of 50 strains of Aeromonas hydrophila, including 28 (56%) strains isolate...

Objective: The study is focused on evaluating the efficacy of seaweed flavonoids against staphylococcus enterotoxin A. Methods: drug-likeness and pharmacological properties chosen ligand were evaluated using SWISS ADME. AutoDockTools-1.5.6 was used to carry out docking analysis determine binding parameters with molecular modeling. Docking visualization performed discovery studio. Results: bioac...

The superantigen staphylococcal enterotoxin A (SEA) requires interaction with class II major histocompatibility complex (MHC) molecules to activate T cells. We have previously used the synthetic peptide approach to establish one side of the hypothetical class II foreign-antigen binding cleft, alpha-helical region 65-85 of the beta chain, as a binding site involved in accessory cell presentation...

The superantigen staphylococcal enterotoxin A (SEA) binds to major histocompatibility complex (MHC) class II molecules at two sites on either side of the peptide groove. Two separate but cooperative interactions to the human class II molecule HLA-DR1 were detected. The first high affinity interaction to the DR1 beta chain is mediated by a zinc atom coordinated by H187, H225, and D227 in SEA and...

The regulation of staphylococcal enterotoxin A (SEA) synthesis in a defined medium was studied using continuous culture techniques. SEA production was repressed by glucose and repression could be overcome by addition of exogenous cyclic AMP. As well as this classical catabolite repression control, addition of glucose to de-repressed steady-state cultures resulted in rapid disappearance of toxin...

OBJECTIVE The aim of this study was to construct a conditionally replicating adenovirus pPE3-SEA expressing staphylococcal enterotoxin A (SEA) gene. MATERIALS AND METHODS A full-length SEA gene fragment was cloned into pENTR12 plasmid to obtain a recombinant viral plasmid pENTR12-SEA. The pENTR12-SEA plasmid was co-transfected into HEK293 cells along with pPE3-ccdB, which encoded for the viru...

  Background : An accurate and prompt diagnosis of bacterial arthritis is essential for earlier treatment and a good outcome. Superantigens produced by Staph. Aureus are among the most lethal toxins. The paper objective was Identification of common bacterial antigens and S.aureus superantigens in synovial fluid (SF) of children with negative culture and direct smear for other bacteria except fo...