Enterotoxigenic Escherichia coli (ETEC) is one of the main causes of illness and death in neonatal and recently weaned pigs. Here, we sequenced the genomes of two ETEC strains that were previously used as inactivated vaccines in China.

Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.

Flooding in Dhaka in July 2004 caused epidemics of diarrhea. Enterotoxigenic Escherichia coli (ETEC) was almost as prevalent as Vibrio cholerae O1 in diarrheal stools. ETEC that produced heat-stable enterotoxin alone was most prevalent, and 78% of strains had colonization factors. Like V. cholerae O1, ETEC can cause epidemic diarrhea.

Enterotoxigenic Escherichia coli (ETEC) strains were readily identified in pure and mixed cultures with nonradioactive, digoxigenin-labeled DNA probes coding for heat-labile (LTI) and heat-stable (STaI, STaII, and STb) enterotoxins. Digoxigenin-labeled ETEC fragments were more sensitive than and exhibited less nonspecific background contamination than biotinylated ETEC probes.

The genetic backgrounds of 24 enterotoxigenic Escherichia coli (ETEC) strains from Mexico and Guatemala expressing heat-stable toxin (ST) and coli surface antigen 6 (CS6) were analyzed. US travelers to these countries and resident children in Guatemala were infected by ETEC strains of sequence type 398, expressing STp and carrying genetically identical CS6 sequences.

From 1996 to 2003, 16 outbreaks of enterotoxigenic Escherichia coli (ETEC) infections in the United States and on cruise ships were confirmed. E. coli serotype O169:H41 was identified in 10 outbreaks and was the only serotype in 6. This serotype was identified in 1 of 21 confirmed ETEC outbreaks before 1996.

Enterotoxigenic Escherichia coli (ETEC) is one of the leading causes of diarrhea in developing countries. Bacteriophage therapy has the potential to aid in the prevention and treatment of ETEC-related illness. To that end, we present here the complete genome of ETEC siphophage Seurat and describe its major features.

Direct detection of enterotoxigenic Escherichia coli (ETEC) in mixed cultures by DNA filter assay was affected by the presence of other bacteria in the cultures. By shortening incubation to 6 h, bacterial overgrowth was minimized, allowing direct detection of ETEC in stools or mixed cultures initially containing 0.2 to 3% ETEC.

The cause of infantile diarrhoea was investigated with the use of Biken test and animal assay for toxigenic bacteria. EnterOtoxigenic Escherichia coli (ETEC) was recovered in 39 cases (17.3%). Of these 30 patients (13.3%) were infected with heat-labile (LT+ETEC) and 7 (3.1%) with heat-stable producing ETEC (ST+ETEC) and 2 (0.9%) with both toxins producing strains (LT+/ST~ETEC). Majority of case...

Results: Diarrhoeagenic E. coli was found in 148 (13.2 %) of examined specimens. Among these 77 (52 %) were enteropathogenic (EPEC), 69 (46.6 %) were enterotoxigenic (ETEC) and 2 (1.4 %) were enteroinvasive Escherichia coli (EIEC). EPEC serotypes 0119K69, 0114K90 and 0111K58 were the more frequent among twelve other serotypes. Diarrhoeagenic Escherichia coli showed high rates of resistance to a...