background: entamoeba moshkovskii and e. dispar are impossible to differentiate microscopically from the pathogenic species e. histolytica . multiplex polymerase chain reaction (multiplex pcr) is a widespread molecular biology technique for amplification of multiple targets in a single pcr experiment. methods: for detection and differentiation of the three-microscopy indistinguishable entamoeba...

BACKGROUND The level of intra-species genetic variation in Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii populations in a localized geographic area, like Puducherry, India, remains unknown. METHODS In the present study the existence of genetic variation in the nested multiplex polymerase chain reaction (NM-PCR) amplified region of the 16S-like ribosomal RNA genes of E. his...

This study aimed to determine the prevalence of Entamoeba histolytica, dispar, and moshkovskii, using microscopic molecular methods in Jahrom city (Fars Province), south Iran. Stool samples were collected from 360 outpatients referred medical laboratories city. Standard parasitological methods, including direct wet mount examination, formalin–ether sedimentation technique, trichrome staining te...

Intestinal amebiasis is a major cause of diarrhea. However, research on host-amebae interactions has been hampered owing to a lack of appropriate animal models. Recently, a mouse model of intestinal amebiasis was established, and using it, we reported that Entamoeba moshkovskii colonized the intestine in a manner similar to that of the pathogenic Entamoeba histolytica In this study, we evaluate...

This is an Open Access article distributed under the terms of the creative commons Attribution 4.0 licence (http://creativecommons.org/licenses/by/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Differentiation of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii from diarrhoeic stools using Polymer...

To address the molecular diversity and occurrence of pathogenic species of the genus Entamoeba spp. in wild non-human primates (NHP) we conducted molecular-phylogenetic analyses on Entamoeba from wild chimpanzees living in the Issa Valley, Tanzania. We compared the sensitivity of molecular [using a genus-specific polymerase chain reaction (PCR)] and coproscopic detection (merthiolate-iodine-for...

Entamoeba moshkovskii cysts are morphologically indistinguishable from those of the disease-causing species E. histolytica and the nonpathogenic E. dispar. Although sporadic cases of human infection with E. moshkovskii have been reported, the organism is considered primarily a free-living amoeba. No simple molecular detection tool is available for diagnosing E. moshkovskii infections. We used p...

The present study was conducted to investigate the clinical outcomes of Entamoeba histolytica infection in symptomatic and asymptomatic Orang Asli (aborigine) communities in Malaysia. Examination was performed on 500 stool samples obtained from Orang Asli communities in 3 different states using formalin-ether concentration, trichrome staining, and single-round PCR techniques. Out of 500 stool s...

Results Out of 274 stool samples, multiplex PCR assay of 61 microscopy positive samples showing cyst and/ trophozoite stage of Entamoeba, revealed a higher prevalence of E. moshkovskii (12.8%; 95% CI= 9.30, 17.28) and lower prevalence of E. dispar (6.2%; 95% CI=3.85, 9.77) compared to E. histolytica (8.1%; 95% CI= 5.31, 11.91; p< 0.05). Of the 61 samples that were microscopically positive, 5 we...

Entamoeba histolytica, the parasitic amoeba responsible for amoebiasis, causes approximately 100,000 deaths every year. There is currently no vaccine against this parasite. We have previously shown that intracecal inoculation of E. histolytica trophozoites leads to chronic and non-healing cecitis in mice. Entamoeba moshkovskii, a closely related amoeba, also causes diarrhea and other intestinal...