This study characterized the fecal indicator bacteria (FIB), including Escherichia coli (E. coli) and Enteroccocus (ENT), disseminated over time in the Bay of Vidy, which is the most contaminated area of Lake Geneva. Sediments were collected from a site located at ∼500 m from the present waste water treatment plant (WWTP) outlet pipe, in front of the former WWTP outlet pipe, which was located a...

The presence, distribution and expression of cassette chromosome recombinase (ccr) genes, which are homologous to the staphylococcal ccrAB genes and are designated ccrAB(Ent) genes, were examined in enterococcal isolates (n=421) representing 13 different species. A total of 118 (28 %) isolates were positive for ccrAB(Ent) genes by PCR, and a number of these were confirmed by Southern hybridizat...

the objective of this study was to evaluate lactic flora identification in fresh (one-day) and ripened (90-day) koozeh cheese samples as one of the raw milk cheeses. in first step, in order to isolate the different genera of lactic acid bacteria (lab), selective and differential (corresponding) media were used as follow: mrs for lactobacilli and pediococci, mrs+ vancomycin for leuconostocs, m17...

The sexpheromone system of Enterococcus faecalis is a form of bacterial conjugation that plays an important role in the horizontal dissemination of genes. The ecological significance of 'sexual' plasmids is to permit a rapid mobilization of genes of interest for the species (e.g. those encoding haemolysins, bacteriocins or antibiotic resistance). The physical mapping of pMB1-1, a conjugative pl...

Six pimarane-type diterpenes isolated from Viguiera arenaria Baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. The microdilution method was used for the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Porphyromonas gingiva...

The fraction of the neutral extraction leaves Espeletia semiglobulata Cuatrec. was subjected to chromatographic separation, it yielded four ent-kaurene type diterpenoids: three known [ent-kaur-16-en-19-al (I), ent-kaur-18-nor-16-en-4-ol (III), ent-kaur-16-en-19-ol (IV)] and a new one elucidated as ent-kaur-3-acetoxy-15-ene (II), based on physicochemical spectroscopic data FTIR, GC-MS, 1D 2D NMR...

Most bacterial source tracking (BST) methods are too expensive for most communities to afford. We developed targeted sampling as a prelude to BST to reduce these costs. We combined targeted sampling with three inexpensive BST methods, Enterococcus speciation, detection of the esp gene, and fluorometry, to confirm the sources of fecal contamination to beaches on Georgia's Jekyll and Sea Islands ...

A waste stabilization pond was suspected of causing fecal contamination to Holt Branch, a tributary of Juniper Creek, near Byron, Georgia. We used ribotyping, a genotypic (DNA-based) bacterial source tracking (BST) method, to match Enterococcus faecalis isolates from Holt Branch and the waste stabilization pond. Fecal enterococci in the effluent from the waste stabilization pond averaged 13,863...

1Department of Microbiology; 2Department of Environmental Health, Isfahan University of Medical Sciences, Isfahan, Islamic Republic of Iran (Correspondence to A. Tavakoli: [email protected]). Received: 05/10/03; accepted: 22/03/04 ABSTRACT To investigate the inactivation of potential pathogens, we evaluated survival rates for Escherichia coli, Enterococcus faecalis, Aeromonas hydrophila and Sa...

A total of 330 lactic acid bacteria isolated from South African red wines during alcoholic and malolactic fermentations and 9 commercial malolactic bacteria starter cultures were screened for antimicrobial activity. Of the entire screened isolates, 26 strains, belonging to the species Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus hilgardii and Oenococcus oeni, showed activity ...