The cell plate of higher plants is formed within a ribosome-excluding cell plate assembly matrix. Phragmoplast microtubules facilitate cell-plate formation by forming a scaffold that directs Golgi-derived vesicles to the forming cell plate. Here, we analyse the effects of the cell-plate assembly matrix on phragmoplast microtubule plus-end geometry by electron tomography of cryogenically fixed A...

The activity and properties of cholinesterase of the motor end plate in human intercostal muscle were studied in the isolated muscle membrane. This preparation was used because cholinesterase activity of the membrane preparation was localized in the motor end plate without contamination of cholinesterase of other muscle components. Under the experimental conditions, cholinesterase in a human en...

End-plate membrane has been examined at amphibian myoneural junctions by means of transmission electron microscopy of thin tissue sections. The postjunctional membrane exhibits morphologically specialized dense, convex patches which are located superficially facing the axon terminal but do not extend into the depths of the junctional folds. In the specialized regions the plasma membrane is appr...

STUDY DESIGN Using fresh cadavers, the biomechanical testing were used to examine the pullout strength of each pedicle screw. OBJECTIVE The aim of this study was to evaluate pullout strength of (1) a redirected pedicle screw following lateral wall breach; (2) a redirected pedicle screw following end-plate breach; and (3) a pedicle without redirection after end-plate breach without redirection...

The reaction kinetics for drug blockade of postsynaptic channels at the mouse neuromuscular junction were studied. The activation energies associated with the onward and off-rate constants, from a sequential model, were measured for procaine, scopolamine, octanol, and heptanol, and from these the transition state thermodynamic parameters of free energy (AG$), enthalpy (AHI), and entropy (ASS) o...

This single fibre EMG study compares the standard method of neuromuscular jitter measurement in voluntarily activated muscle to that by intramuscular electrical stimulation of motor axons in a group of normal subjects. The latter method avoids the interdischarge interval-dependent jitter, as well as a possible failure to recognise split muscle fibres. The mean MCD on axonal stimulation was only...

IT HAS BEEN CONSIDERED that the end-plate potential (e.p.p.) is generated by the brief ionic flux across the end-plate membrane and the later slowly declining phase of the e.p.p. is due to the dissipation of the charge along and across the muscle membrane. This consideration was supported by some authors. Kuffler (21) observed with a single nerve-muscle preparation that the later slowly decayin...