This study was designed to determine the response of diverse mint genotypes to three commonly used cryopreservation techniques. Four mints [Mentha x piperita nothosubsp. citrata (Ehrh.) Briq.; M. canadensis L.; M. australis R. Br, and M. cunninghamii Benth] were cryopreserved using three protocols: controlled rate cooling (CC), encapsulation dehydration (ED) and PVS2 vitrification (VIT). Regrow...

Cryopreservation has been proven to be an effective technology for the cost-effective, longterm preservation of genetic material. A wide range of plant material including cultured cells, tissues, embryos, meristems, pollen and seeds can be effectively preserved for extended periods of time and, when thawed, can be used to rapidly produce stock plants, with good preservation of genetic and physi...

Cryopreservation of germplasm in liquid nitrogen (LN) is a perfect method for the long-term conservation of plant genetic resources. Lily [Lilium ledebourii (Baker) Bioss.], Persian lilac (Melia azedarach L.), and tea (Camellia sinensis L.) were evaluated for long-term storage in liquid nitrogen (LN) at -196oC. Encapsulation within alginate beads was shown to be beneficial in all studied specie...

Embryogenic cell suspensions of two elite Spanish grapevine cultivars (‘Albariño’ and ‘Tempranillo’) were successfully cryopreserved by encapsulation-dehydration. The method implied the encapsulation of cells in alginate beads, subsequent culture in liquid medium with 1 M sucrose for 4 days, and desiccation for 2-4 h in the flow of a laminar-flow bench, before immersing in liquid nitrogen. With...

Abstract. Seeds or plant organs are usually used as the materials for the long-term cryopreservation. The aim of this study is to investigate the possibility of seed cryopreservation of Lilium ledebourii (Baker) Boiss. as an endemic and endangered species because of genetic erosion. To evaluate seed potentials for the cryopreservation, four treatments including vitrification, 30% glycerol, desi...