the aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. blastocyst stage embryos from pregnant nmri mice were obtained and cultured for 1-2 days in dmem medium. the inner cell masses formed...

bone tissue engineering has been one of the most promising areas of research, providing a potential clinical application to cure bone defects. recently, various stem cells, including embryonic stem cells (escs), bone marrow-derived mesenchymal stem cells (bm-mscs), umbilical cord blood-derived mesenchymal stem cells (ucb-mscs), adipose tissue-derived stem cells (adscs), muscle-derived stem cell...

background  the mammary gland in humans is a dynamic organ that undergoes significant developmental changes during pregnancy, lactation, and involution. stem cells derived from human breast milk possess the adult stem cell-like characteristics such as self-renewal, proliferative and differentiate potential. this source of stem cells avoids invasive procedures and the ethical controversy of embr...

introduction: type i diabetes mellitus is caused by autoimmune destruction of the insulin-producing β-cells. a new potential method for curing the disease is transplantation of differentiated insulin- secreting cells from human embryonic stem cells. methods: human embryonic stem cell lines (royan h1) were used to produce embryoid bodies. differentiation carried out by growth factor-mediated sel...

background: embryonic stem (es) cells are pluripotent cells conventionally isolated from early embryos. studies have shown that es cells serve as a practical model for biomedical studies. objective: the aim of the present study was to optimize culture conditions for establishment of es-like colonies from nmri mouse blastocysts as well as 2-cell stage embryos. materials and methods: both expande...

background: creation of artificial gametes may provide a universal solution for these patients of lacking gametes. stem cell technology may provide a way to obtain fully functional gametes. retinoic acid (ra) can initiate meiosis. several studies have demonstrated that ra can promote sperm cells differentiation from mouse embryonic stem cells (mescs) and other cells from human embryonic stem ce...

Background: We have recently shown that human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs) can differentiate into advanced spermatogenic cells including round spermatids by in vitro culture (Easley et al., Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells. Cell Reports 2, 440-446 2012) and also, in collaboration, that rhesus spermatogonial ...

abstract introduction: cancer stem cells are a small subpopulation of cells within a tumor which are responsible for maintaining the tumor mass. a number of factors such as oct-4 that govern the fate of adult stem cells also play a role in malignant cell transformation. oct-4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis ...

The possibility to reprogram somatic human cells will greatly and deeply change genetic approach and allow the development of new tools to study genetics diseases. Indeed, our ability to study human genetic diseases suffers from the lack of valid in vitro models. The latter should (i) be originating from human primary cells, (ii) be able to self-renew for a long time and (iii) be able to differ...