Background: Because of the problems of achieving to mature oocytes, abillity to deliver the oocytes to mature outside of ovaries was a big discovery that somewhat could reduce the risk of ART`s.Because of the low percentage of in vitro oocyte maturation, research for improving the IVM condition and increasing the oocyte maturation rate is to be continued. The aim of this research is the study o...

background to current knowledge, different oocyte's recovery method and various seasons have profound impact on in vitro embryo production (ivep). objectives the aim of this study was to define an efficient recovery method for oocytes harvesting from slaughterhouse material in different seasons, and their effects on ivep yield. materials and methods ovaries from slaughtered ewes in breeding sea...

background: it is important to protect oocytes and embryos from oxidative stress in the culture medium. melatonin has been shown to be a direct free radical scavenger. objective: effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. materials and methods: oocytes from supper-ovulated mouse were divided to two groups: cumulus ...

background: linoleic acid (la) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence.objective: in the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovinematerials and methods: the experime...

In the exalbuminous species Arabidopsis thaliana, seed maturation is accompanied by the deposition of oil and storage proteins and the reduction of the endosperm to one cell layer. Here, we consider reserve partitioning between embryo and endosperm compartments. The pattern of deposition, final amount, and composition of these reserves differ between the two compartments, with the embryo repres...

background: ammonium is produced in culture medium due to amino acids degradation and has adverse effect on in vitro culture of embryo. in the current study, the purpose was to evaluate the effects of ammuniom chloride (ac) on in vitro oocyte maturation rate and early embryo development in the sheep and its effect on the expression of bcl-2. methods: in vitro maturation (ivm) was performed in t...

introduction: standard concentrations of antibiotics in culture media are thought to have no detectable toxic effects on the cultured cells. however, since antibiotics are biologically active substances, the possibility that they interfere to some extent with cellular processes occurring in the cultured cells can not always be totally excluded. this study, therefore, was conducted to assess whe...

Background: It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger. Therefore, effect of melatonin during in vitro oocyte maturation (IVM), fertilization and embryo development of mouse oocytes was evaluated. Materials and Methods: Oocytes from supper-ovulated mouse were divided to two groups: cu...

Background: The efficiency of in vitro maturation (IVM) has remained low despite extensive effort to understand the culture conditions. This study investigated the effect of cumulus cells and meiotic inhibitors on oocyte developmental competence in the presence of Alpha Lipoic acid (ALA) Materials and Methods: Mouse germinal vesicle oocytes were considered in cumulus-denuded oocytes (CDOs) and ...