1. The liver of the domestric fowl (Gallus gallus) remains capable of conjugating o-aminophenol with glucuronic acid after 8 days' culture. The pathway of o-aminophenyl glucuronide formation in cultured liver, as in fresh tissue, includes the enzyme UDP-glucuronyltransferase. 2. UDP-glucuronyltransferase activity in chick-embryo liver increases on culture from very low to adult values within 6-...

Objective(s): In veterinary medicine, zeolites are used as toxin binder in animal feed, ammonia purification of aquatic pools, animal smell and moisture control. This study investigated on the risk assessment of nano zeolite on development of embryonic chicken models, anomalies, tetratogenic and embryotoxic effects. Methods: Eggs (120 = n) were accidentally di...

This study investigated whether the same cytochrome P-450 (P-450) isoenzymes were inducible in cultures of chick-embryo hepatocytes as in the liver of chicken embryos. We purified two isoenzymes of cytochrome P-450 from the livers of 17-day-old-chick embryos: one of molecular mass approx. 50 kDa induced in vivo by the phenobarbital-like inducer glutethimide, and the second of approx. 57 kDa ind...

Research Question: Does in vitro exposure of preimplantation mouse embryos to the ketone bodies β-hydroxybutyrate (βOHB) and acetoacetate (AcAc) impact post-transfer fetal placental gene expression?DesignBlastocysts cultured with or without 2 mmol/L βOHB alone (‘βOHB’) combined 0.8 AcAc (‘Keto’) underwent embryo transfer. Transcriptional profiles sexed E14.5 placentae, liver, brain were examine...

A CONSIDERABLE amount of work has been done on the growth and regeneration of the adult mammal liver and theories of liver growth control have been put forward, the most far-reaching of which was advanced by Glinos (1958). According to this theory, the growth of the liver is regulated by a feed-back mechanism, involving diffusible chemical substances secreted into the blood by the liver itself....

Introduction The molecular mechanisms involved in the regulation of hepatic microsomal UDP-glucuronyltransferases are currently the subject of rigorous investigations by a number of laboratories. Overwhelming evidence indicates that UDPglucuronyltransferase is a heterogeneous family of enzymes (see Burchell, 1981); thus these investigations in mammalian tissues are complicated by the specificit...

The rapid incorporation of labeled carbon dioxide into succinate of rat, liver in viva was previously demonstrated (1). In these experiments, the time of sacrifice after a single injection of the isotope was less than 30 minutes, and the experimental approach involved isolation of certain intermediates of the Krebs cycle. In whole homogenates (2), a similar rapid conversion of carbon dioxide to...

Abstract Kupffer cells (KCs) are the resident macrophages of liver with similar origins to myeloid-derived macrophages. Once differentiated, KCs exhibit distinct cellular machinery capable longevity and self-renewal, making them a crucial player in promoting effective intrahepatic communication. However, this gets compromised disease states like Nonalcoholic Steatohepatitis (NASH), where loss e...

1. A method is described for the primary growth of chick embryo liver cells on cover slips in culture, and the factors of the culture medium are considered that affect the induction of porphyrin formation with certain chemicals. 2. A method is described for following &aminolevulinic acid synthetase (ALA synthetase) activity by the determination of porphyrin fluorescence developed in the cells o...