Acute rejection remains an important problem after kidney transplantation. Enzyme-linked immunosorbent spot (ELISPOT) assay has been investigated extensively and shown promising results as a predictor of allograft rejection. The objective this study was to systematically review analyze the predictive value donor-specific ELISPOT identify recipients at risk for acute Electronic databases were se...

BACKGROUND Despite recent advances in cancer immunotherapy and the development of various assays for T cell assessment, a lack of universal standards within immune monitoring remains. The objective of this study was to evaluate the enzyme-linked immunosorbent spot (ELISpot) assay in comparison with major histocompatibility complex-tetramer analysis in the context of dendritic cell (DC)-based ca...

BACKGROUND Currently, reliable efficacy markers for assessment of new interventions against tuberculosis (TB) are limited to disease and death. More precise measurement of the human immune response to Mycobacterium tuberculosis infection may be important. A qualitative enzyme-linked immunospot assay (ELISPOT) result for early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10...

Latent tuberculosis infection (LTBI) is defined as an infection with Mycobacterium tuberculosis (MTB) without clinical, bacteriological, or radiological findings, and its early diagnosis is essential for eradication of tuberculosis. To identify LTBI, we measured the numbers of interferon-γ producing T cells, based on the ELISPOT assay, and the antibody titers in the sera to tuberculous glycolip...

 Nitrocellulose or PVDF? There are two different types of membranes (in 96-Well plate formats) that are used in ELISPOT applications: Nitrocellulose (NC) and polyvinylidene fluoride (PVDF). The reason that these are the predominant membrane types used in ELISPOT is based largely on their fortuitous suitability in non-ELISPOT applications rather than on the development of optimized assay substr...

results almost 8% of the personnel showed positive tst (over 10 mm) reaction while 29% of them had considerable t - cell reactivity against ppd in elispot assays. however, the esat - 6 reactivity was found only in one case of hcws. no correlation was found between the patterns of the reactions and the age or the duration of the employment or previous vaccination history of the participants. the...

Enzyme-linked immunospot, or ELISPOT, assay allows the detection of low frequencies of cells secreting various molecules. ELISPOT can be used in many areas of research and, because of its high sensitivity, has the potential to become a valuable diagnostic tool. Based on the same "sandwich" immunochemical principles as enzyme-linked immunosorbent assay, ELISPOT is easy to perform and quantify th...

BACKGROUND Repeat tuberculin skin tests may be false positive due to boosting of waned immunity to past mycobacterial exposure. We evaluated whether an ELISPOT test could identify tuberculosis (TB) contacts with boosting of immunity to non-tuberculous mycobacterial exposure. METHODOLOGY/PRINCIPAL FINDINGS We conducted tuberculin and ELISPOT tests in 1665 TB contacts: 799 were tuberculin test ...

PURPOSE To evaluate the diagnostic utility and predictors for determinate results of an enzyme-linked immunospot assay using induced sputum cells (IS ELISPOT) for a rapid diagnosis of pulmonary tuberculosis (TB). MATERIALS AND METHODS Subjects suspected of pulmonary TB who had either sputum acid fast bacilli smear-negative or not producing sputum spontaneously were prospectively enrolled. ELI...

Micron scale latex beads are well established as highly biocompatible reagents. Imbibing two fluorescent dyes into the interior of the beads enables the creation of a family of combinatorially colored labels. Previous use of such beads, in flow cytometry for example, has focused on beads of approximately 5 microm diameter. We show here that 280 nm combinatorially labeled particles can be used t...