Hypericumperforatum (H. perforatum) ethanol extract has been found to inhibit lipopolysaccharide-induced production of inflammatory mediators and cytokines in cultured macrophages. Therefore, it may be able to protect the host from excessive inflammation during viral infection. In the current study, the immune-regulatory effect of H. perforatum extract was evaluated in A549 lung epithelial cell...

Host resistance in Desi and commercial layer breeds was studied in this study. It was evaluated by measuring sensitivity of RBCs to LPAIV induced hemagglutination by using RBCs of two breeds and nine other avian species. Moreover, egg infectious dose 50 (EID50) along with mean death time (MDT) in embryonated eggs, intracerebral pathogenicity in dayold chicks, and intravenous pathogenicity, in f...

Thermal inactivation of the H5N1 high pathogenicity avian influenza (HPAI) virus strain A/chicken/Korea/ES/2003 (Korea/03) was quantitatively measured in thigh and breast meat harvested from infected chickens. The Korea/03 titers were recorded as the mean embryo infectious dose (EID50) and were 10(8.0) EID50/g in uncooked thigh samples and 10(7.5) EID50/g in uncooked breast samples. Survival cu...

In 2014-2015, the US experienced an unprecedented outbreak of H5 clade 2.3.4.4 highly pathogenic avian influenza (HPAI) virus. The H5N2 HPAI virus outbreak in the Midwest in 2015 affected commercial turkey and layer farms, but not broiler farms. To assess any potential genetic resistance of broilers and/or age-related effects, we investigated the pathogenesis and transmission of A/turkey/Minnes...

Mammalian cells utilize a wide spectrum of pathways to antagonize the viral replication. These are typically regulated by antiviral proteins and can be constitutively expressed but also exacerbated interferon induction. A myriad interferon-stimulated genes (ISGs) have been identified in mounting broad-spectrum responses. Members interferon-induced transmembrane (IFITM) family unique among these...

BACKGROUND From December 2014 through June 2015, the US experienced the most costly highly pathogenic avian influenza (HPAI) outbreak to date. Most cases in commercial poultry were caused by an H5N2 strain which was a reassortant with 5 Eurasian lineage genes, including a clade 2.3.4.4 goose/Guangdong/1996 lineage hemagglutinin, and 3 genes from North American wild waterfowl low pathogenicity a...

Avian infectious bronchitis virus (IBV) causes tremendous economic losses to the poultry industry worldwide. Different serotypes of this virus show little cross-protection. The present study investigated the relationship between differences in the genotype based on the N-terminus of the spike protein and the protection provided by vaccination with IBVs. Cross-immunization tests were performed u...

Five commercial LaSota strain Newcastle disease (ND) vaccines namely A, B, C, D and E were evaluated for their potency, efficacy, thermostability and influence on productivity in broilers. A 3-log10 difference of EID50 and two-to-eight fold difference of HA activity was found among the various vaccines tested. One hundred and fifty day-old broiler chicks were divided into six equal groups tagge...

The aim of the study was to find out the host specificity of pigeon pox virus-1 (PPV-1) and fowl pox virus-2 (FPV-2) following cross infection with field strains from Bangladesh. The cross infection was investigated in five groups (n=5) of pigeons and seven groups of chickens (n=5) four weeks of age. Irrespective of route of inoculation each bird received either 0.1 mL of PPV-1 or FPV-2 having ...