White leghorn hens were immunized intramuscularly with formalin killed Klebsiella pneumoniae cells to generate Klebsiella pneumoniae antibodies (IgY) in egg yolk. Booster injections of increasing concentrations of antigen were given to raise the antibody level in egg yolk. Amount of protein varied from 0.87 7.4mg/ml in egg yolk throughout the immunization period. The antibodies were partially p...

The aim of this study was to characterize of chicken egg yolk immunoglobulins (IgYs) specific as immunotherapy to Mycobacterium tuberculosis complex (MTBC) infection. Lohmann laying hens were immunized intramuscularly with antigenic of MTBC. Egg yolk was separated from egg white, and IgY antibody was then purified by multiple polyethylene glycols 6000 extraction and ammonium sulfate purificatio...

background : staphylococcus aureus strains exhibiting multiple antibiotic resistances are isolated from most communities and hospital infections. treatment of patients with these infections has been difficult. the aim of this study was to detect and extract, the egg yolk immunoglobulin y as a potential source of anti- s. aureus antibody. methods : specific igy was produced by immunizing hens wi...

Avian embryos and neonates acquire passive immunity by transferring maternal immunoglobulins from serum to egg yolk. Despite being a convenient source of antibodies, egg yolk immunoglobulins (IgY) from immunized hens have so far received scant attention in research. Here we report the generation and rapid isolation of IgY from the egg yolk of hens immunized against the alpha subunit of the huma...

Maternal antibodies protect chicks from infection with pathogens early in life and may impact pathogen dynamics due to the alteration of the proportion of susceptible individuals in a population. We investigated the transfer of maternal antibodies against avian influenza virus (AIV) in a key AIV host species, the mallard (Anas platyrhynchos). Combining observations in both the field and in mall...

Indirect enzyme linked immunosorbent assay was developed to measure specific antibody activity to outer membrane proteins in sera and egg yolk of chickens exposed to Salmonella Gallinarum infection and also following vaccination. The observed end point titers were used to formulate regression equation and the absorbance data obtained at a single serum dilution were converted directly to antibod...

one-hundred-sixty-eight 28-wk-old hy-line layers (w-36) were used to evaluate the effect of different levels of linseed (ls) and safflower seed (ss) on antibody titter, blood and yolk cholesterol, yolk fatty acid content and the ratio of omega-6/ omega-3. hens were randomly assaigned in a compeletly randomised design each 3 replicates of 8 hens. they were fed diets contained 0, 4, 7 and 10% ls ...

The production of anti-snake venom from large mammal's blood has been found to be low-yielding and arduous, consequently, antivenom immunoglobulins for treatment are achieved regularly as polyvalent serum. We have standardized an undemanding technique for making purified immunoglobulin IgY antivenom consisting of polyclonal antibodies against coral snake venom in the egg yolk of immunized hens....