To investigate E7-dependent biochemical changes which are involved in cellular transformation, we analyzed the influence of human papillomavirus type 16 (HPV-16) E7 on the expression of cell cycle regulatory proteins. Expression of E7 in established rodent fibroblasts (NIH 3T3), which was shown to be sufficient for transformation of these cells, leads to constitutive expression of the cyclin E ...

A synthetic E7 gene of human papillomavirus (HPV) type 16 was generated that consists entirely of preferred human codons. Expression analysis of the synthetic E7 gene in human and animal cells showed levels of E7 protein 20- to 100-fold higher than those obtained with wild-type E7. Enhanced expression of E7 protein resulted from highly efficient translation, as well as increased stability of th...

Background: Vaccines capable of controlling tumor virus based infections are found difficult to develop due to the consistence latent infection in the host. DNA vaccines are attractive tools for the development of HPV vaccines and inducing antigen-specific immunity owing to the stability, simplicity of delivery, safety and cost effectiveness. However, there is a need to increase their potency b...

background: vaccines capable of controlling tumor virus based infections are found difficult to develop due to the consistence latent infection in the host. dna vaccines are attractive tools for the development of hpv vaccines and inducing antigen-specific immunity owing to the stability, simplicity of delivery, safety and cost effectiveness. however, there is a need to increase their potency b...

Background: Cervical cancer is the most prevalent tumor in developing countries and the second most frequent cancer among female population worldwide. Specific human papillomaviruses and, most notably, HPV types 16 and 18 are recognized as being caus-ally associated with cervical carcinomas. The early HPV type 16 genes, E6 and E7, di-rectly participate in the in vitro transformation of primary ...

the development of a therapeutic vaccine against human papillomavirus (hpv) is important for the control of cervical cancer. e7 is the major transforming protein produced in cervical cancers, and therefore represents potential tumor-specific antigen that could be the target of immunotherapy for cervical cancer. among different vaccine strategies, protein-based vaccines are capable of generating...

The soybean lectin gene Lel encodes a prevalent seed protein and is highly regulated during the life cycle. The mutant lectin gene allele /e7 is not transcribed detectably, contains a 3.541 Tgml insertion element within its coding region 0.6 kb 3' to the transcription start site, and leads to a lectinless phenotype. To determine whether the Tgml element or a secondary mutation was responsible f...

objective: dna vaccines have been widely used to develop immunity against various pathogens including parasites and viruses. the potential of dna vaccine to induce an effective immune response is related to the expression levels of the encoded protein in eukaryotic cells. therefore, optimization of plasmid dna delivery system is a major concern in protein expression in order to make an efficien...

Recently, self-replicating RNA vaccines (RNA replicons) have emerged as an effective strategy for nucleic acid vaccine development. Unlike naked DNA vaccines, RNA replicons eventually cause lysis of transfected cells and therefore do not raise the concern of integration into the host genome. We evaluated the effect of linking human papillomavirus type 16 E7 as a model Ag to Mycobacterium tuberc...

background: toll like receptors (tlrs) are one of the main components of the innate immune system. it has been reported that expression of these receptors are altered in the female reproductive tract (frt) during menstrual cycle. here we used a fallopian tube epithelial cell line (oe-e6/e7) to evaluate the effect of two sex hormones in modulating tlr expression. materials and methods: in this e...