Two possible isomers of some 1,3-diaryl-H-benzo[f]chromene have been studied using density functional theory. Structures of E1 and E2 isomers were optimized at the B3LYP and MP2 levels with different basis sets. The total electronic energies show that E2 isomer is about 3-5 kcal/mol more stable than E1 isomer and this energy difference is attributed to the planarity of heterocyclic ring and mor...

Introduction: The development of an effective vaccine against Hepatitis C virus (HCV) is still a target of intensive vaccine research. The HCV envelope proteins E1 and E2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. Different types of expression systems have been used to express these glycoproteins. In this study, an expression system using Pichia p...

ion and function application employ the notion of substitution. Recall the inference rule and its representation in LF: e1 ↪→ lam x. e1 e2 ↪→ v2 [v2/x]e1 ↪→ v ev app e1 e2 ↪→ v ev app : ΠE1:exp. ΠE2:exp. ΠE′ 1:exp→ exp. ΠV2:exp. ΠV :exp. eval E1 (lam E′ 1) → eval E2 V2 → eval (E′ 1 V2) V → eval (app E1 E2) V. As before, we transcribe this (and the trivial rule for evaluating λ-expressions) into...

Prolactin (PRL) has numerous physiological functions that are mediated by its receptors in target cells. Expression of the rat PRL receptor (PRLR) gene is regulated in a tissue-specific manner via the transcriptional activation of five distinct first exons, i.e., E1(1), E1(2), E1(3), E1(4), and E1(5). In the present study, we investigated the expression profiles of these first exon variants of ...

In this study, a screening of the efficacy microbial consortium bacteria and fungi isolated from activated sludge, river sediment, compost for degradation LDPE/TPS was performed. According to morphological biochemical characterization, eight bacteria, Bacillus sonorensis, subtilis, Lysinibacillus massiliensis, licheniformis, indicus, megaterium, cereus, Pseudomonas alcaligenes, five molds, Aspe...

Human E1 is a key player in protein ubiquitination, however the E1 structure is not available. In this paper, we describe the derivation of a human E1 structure using molecular modelling based on the crystal structure of S. cerevisiae E1 and M. Musculus E1. Key interactions between our E1 model and ubiquitin are also discussed.

KCNE1 (E1) β-subunits assemble with KCNQ1 (Q1) voltage-gated K(+) channel α-subunits to form IKslow (IKs) channels in the heart and ear. The number of E1 subunits in IKs channels has been an issue of ongoing debate. Here, we use single-molecule spectroscopy to demonstrate that surface IKs channels with human subunits contain two E1 and four Q1 subunits. This stoichiometry does not vary. Thus, I...