We sequenced exon 2 of the major histocompatibility complex (MHC) class II DRB3 gene from 471 individuals in four different Japanese populations of cattle (201 Japanese Black, 101 Holstein, 100 Japanese Shorthorn, and 69 Jersey cattle) using a new method for sequence-based typing (SBT). We identified the 34 previously reported alleles and four novel alleles. These alleles were 80.0-100.0% ident...

Background: Polycystic Ovarian Syndrome (PCOS) is a pro-inflammatory condition that supports the development of metabolic aberration and ovarian dysfunction. Chronic inflammation and the increased levels of androgens in this group of patients and their impact on the immune system may increase the risk of developing malignancies, including ovarian cancer. Thus, we interacted ovarian tumor cells ...

The present study was conducted to study the diversity of MHC-DRB3 alleles in Indian cattle and buffalo breeds. Previously reported BoLA-DRB exon 2 alleles of Indian Zebu cattle, Bos taurus cattle, buffalo, sheep, and goats were analyzed for the identities and divergence among various allele sequences. Comparison of predicted amino acid residues of DRB3 exon 2 alleles with similar alleles from ...

background: there is a worldwide interest in searching for potential and effective medicinal plant candidates against various diseases or disorders. till now, there are number of anti-inflammatory drugs like nsaids are available which showed various adverse effects in our body. to reduce these adverse effects, some of medicinal plants have been experimentally validated. aim: the aim of our immu...

The MHC of cattle, known as the bovine leukocyte antigen (BoLA) complex, plays an integral role in disease and parasite susceptibility, and immune responsiveness of the host. While susceptibility to tick infestation in cattle is believed to be heritable, genes that may be responsible for the manifestation of this phenotype remain elusive. In an effort to analyze the role that genes within the B...

atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by innate and adaptive immune responses to a variety of microbial and self-antigens. given the crucial role of adaptive immunity in the pathogenesis of atherosclerosis, this study was performed to investigate the proliferative response of peripheral blood mononuclear cells (pbmc) and interleukin (il)-2 producti...

PROBLEM The regulatory mechanisms governing differential expression of classical major histocompatibility complex (MHC) class I (MHC-Ia) and non-classical MHC class I (MHC-Ib) genes are poorly understood. METHOD OF STUDY Quantitative reverse transcription- polymerase chain reaction (PCR) was used to compare the abundance of MHC-I transcripts and related transcription factors in peripheral blo...

silybum marianum, is known to have anti-inflammatory, hepatoprotective and anticarciogenic effects. the aim of this study was to compare effects of silymarin, rapamycin and fk506 on proliferation and apoptosis of human t cells stimulated with con a. peripheral blood mononuclear cells (pbmc) were stimulated with concavalin a  ( con a) (5µg/ml) and then treated with different inhibitors (silymari...

glioblastoma multiforme and other malignant gliomas continue to be associated with a dismal prognosis despite advances in diagnosis and therapy.1,2 Mean survival from the time of diagnosis is less than one year in most cases. This has spurred investigation of new treatment modalities such as immunogene ABSTRACT: Background: Cancer immunogene therapy is based on vaccination with radiated, autolo...

Objective. To determine the associations of BoLA DRB3.2 alleles present in Holstein and BON x Holstein cattle to production and milk quality traits in a dairy herd of Antioquia, Colombia. Materials and methods. Ninety-one cows, 66 Holstein and 25 BxH, were genotyped for the BoLA DRB3.2 gene, through PCR-RFLP technique. Furthermore, the association of the alleles of the gene BoLA DRB3.2 with mil...