Aim: Diabetes mellitus (DM) is an important health problem with increasing incidence worldwide and causes many complications. Diabetic retinopathy (DR) one of the most serious complications DM. Polymorphisms AKR1B1 gene, which encodes aldose reductase enzyme, have been associated development DM DR in some studies. The current study aims to investigate relationship rs759853 polymorphism type 2 (...

In search of an effective DNA typing technique for Acinetobacter baumannii strains for hospital epidemiology use, the performance and convenience of a new target sequence was evaluated. Using known genomic sequences of Acinetobacter baumannii strains AR 319754 and ATCC 17978, we developed single-locus variable-number direct-repeat analysis using polymerase chain reaction-restriction fragment le...

Latar Belakang : Stroke merupakan penyebab utama kematian dan kecacatan di dunia. Terjadinya stroke iskemik dipengaruhi oleh faktor genetik, lingkungan interaksi keduanya. Variasi genetik gen Angiotensinogen (AGT) M235T berhubungan dengan hipertensi diabetes mellitus yang resiko dari stroke.
 Tujuan Mengidentifikasi variasi pada penderita Iskemik Rumah Sakit Umum Pusat Dr. Kariadi, Semaran...

The direct repeat (DR) region has been determined to be an important chromosomal domain for studying the evolution of Mycobacterium tuberculosis. Despite this, very little is known about microevolutionary events associated with clonal expansion and how such events influence the interpretation of both restriction fragment length polymorphism (RFLP) and spoligotype data. This study examined the s...

background:diabetic retinopathy (dr) is a sight-threatening microvascular complication of diabetes in which the vascular endothelium is damaged due to oxidative stress and inflammation, and vitreous vegf concentration becomes elevated. the aim of the present study was to assess the association of dr with genetic variations of the mnsod, a major antioxidant enzyme, and vegf, an important mediato...

Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and a PCR-based typing method called spacer oligonucleotide typing (spoligotyping) were used to strain type Mycobacterium bovis isolates from the Republic of Ireland. Results were assessed for 452 isolates which w...

OBJECTIVES To study if the reported association of a BamH I 2kb RFLP of the T cell receptor V beta 8 gene with DR4+ rheumatoid arthritis patients is found in non-American white populations. METHODS The frequency of this RFLP in two different populations was analysed. Eighty one northern Italians were studied for HLA-DR genotypes and V beta 8 polymorphism, and 29 DR4+ British white patients we...

Objective
 Type 2 diabetes mellitus (T2DM) and diabetic
 retinopathy (DR) development is affected by genetic
 factors. Matrix metalloproteinases (MMPs) are involved
 in many cellular processes, such as remodeling of
 extracellular matrix proteins angiogenesis. In
 this study, we investigated the relationship between
 MMP9 −1562 C/T (rs3918242) polymorphism the...

From May to November 2005, a study was carried out to assess the occurrence of trypanocidal drug resistance (DR) in trypanosomes of naturally infected cattle of the Adamaoua region of Cameroon. Two distinct Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) procedures were used together with an Allele specific-PCR (AS-PCR) and the standardized single-dose mouse test. ...

Spoligotyping (for spacer oligotyping) is an easy, economical, and rapid way of typing Mycobacterium tuberculosis complex strains with the DR spacer markers (J. Kamerbeek et al., J. Clin. Microbiol. 35:907-914, 1997; D. van Soolingen et al., 33:3234-3248, 1995). The stability of the markers was demonstrated by showing that all the Mycobacterium bovis BCG strains tested gave the same spoligotypi...