نتایج جستجو برای: down pcr
تعداد نتایج: 377022 فیلتر نتایج به سال:
this study investigated (a) the learners’ existing reading strategy repertoire, (b) the effect of instruction in reading strategies on learners’ strategic performance, and (c) the effect of explicit instruction in top-down reading strategies on reading comprehension ability of intermediate learners. the study was conducted with 40 intermediate efl learners in two groups of experimental and cont...
Purpose: Effect of lipocalin 2 on the expression of heme oxygenase I , II and NF-kB transcription factor was the purpose of this survey. Materials and Methods: Lcn2 was cloned to pcDNA3.1 plasmid by using genetic engineering method. The recombinant vector was transfected to CHO and HEK293T to establish stable cell expressing lipocalin 2. The presence of lipocalin 2 gene in these cells was confi...
Background: OCT4B1, a variant of OCT4, is expressed in both cancer cells and tissues. This variant has a main role in the regulation of both apoptotic and stress (heat-shock proteins) pathways. The aim of this study was to investigate the effects of OCT4B1 silencing on the expression of small heat-shock proteins (sHSPs)in three human tumor cell lines. Methods:...
Abstract Background Long intergenic non-protein coding RNA 00342 (LINC00342) has been identified as a novel oncogene. However, the functional role of LINC00342 in colorectal cancer (CRC) remains unclear. Methods The expression is detected by real-time PCR (RT-PCR) analysis. Cell proliferation, migration and invasion xenograft model are examined to analyze biological functions vitro vivo using c...
miR-499a-5p has been reported to regulate the progression of various tumours. However, role in breast cancer is unclear. The purpose this study was investigate and mechanism cancer. growth effect on cells investigated by CCK-8 assay, wound healing assay Transwell invasion assay. luciferase activity used verify downstream targets miR-499a-5p. levels GSH, MDA ROS were detected kits. Quantitative ...
objective(s):eukaryotic translation initiation factor 4e (eif4e) is overexpressed in cervical cancer (cc). however, the molecular mechanisms are unclear. this study aimed to investigate the molecular mechanism of eif4e gene overexpression in cc. materials andmethods:the human papillomavirus (hpv) type 18 e7 and eif4e mrnas were measured following knock down or overexpression of e7 gene by rt-pc...
objective: in this study, the possibility of prenatal diagnosis of down syndrome with real-time pcr method was evaluated. in this context, optimization of a suitable method for purification of high quality dna from amniotic fluid samples was also considered. materials and methods: pregnant women who had the high risk of having babies with down syndrome were selected according to the biochemica...
The miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer
Objective(s): To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). Materials and Methods: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate produc...
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