haploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter- and intraspecific via chromosome elimination. Here, we fo...

to enhance germplasms of bread wheat, a gene, encoding the smallest y-type high molecular weight glutenin subunit (hmw gs) gene (12.4t) from the d-genome using synthetic hexaploid approach was introgressed into bread wheat. tetraploid wheat (t. turgidum cv. langdon) was crossed with t. tauschii accession aus24092. immature embryos were rescued and placed onto b5 regeneration media. hybrid f1 pl...

pod length is one of the effective components on canola yield selection on the basis of which can increase not only the seed yield but also the oil yield. molecular markers asso-ciated with long pod loci were identified in a doubled haploid population derived from a cross between the canola lines ‘quantum’ (long pod) ‘china a’ (short pod) using rapd and bulked segregant analysis. a molecular ma...

Chrysanthemum is one of important ornamental species in the world. Its highly heterozygous state complicates molecular analysis, so it is of interest to derive haploid forms. A total of 2579 non-fertilized chrysanthemum ovules pollinated by Argyranthemum frutescens were cultured in vitro to isolate haploid progeny. One single regenerant emerged from each of three of the 105 calli produced. Chro...

Doubled haploid technology has become an increasingly popular and important tool for developing new maize lines (Zea mays L.) breeding. Conventional breeding requires repeated self-pollination about six to ten generations obtain homozygous inbred lines. Completely can be produced in only two by using doubled technology. In vivo induction of the maternal method was used production 32 different d...

Anther culture-derived haploid embryos were used as explants for Agrobacterium-mediated genetic transformation of bread wheat (Triticum aestivum L. cv CPAN1676) using barley HVA1 gene for drought tolerance. Regenerated plantlets were checked for transgene integration in T₀ generation, and positive transgenic haploid plants were doubled by colchicine treatment. Stable transgenic doubled haploid ...